Amentoflavone Induces Apoptosis and Inhibits NF-ĸB-modulated Anti-apoptotic Signaling in Glioblastoma Cells

Tsung-Hsien Yen, Chia-Ling Hsieh, Tsu-Te Liu, Chih-Sheng Huang, Yen-Chung Chen, Yao-Chen Chuang, Song-Shei Lin, Fei-Ting Hsu

研究成果: 雜誌貢獻文章

5 引文 (Scopus)

摘要

The goal of the present study was to investigate anticancer effect of amentoflavone on glioblastoma cells in vitro. Our results demonstrated that amentoflavone not only significantly reduced cell viability, nuclear factor-ĸappa B (NF-ĸB) activation, and protein expression of cellular Fas-associated protein with death domain-like interleukin 1 beta-converting enzyme inhibitory protein (C-FLIP) and myeloid cell leukemia 1 (MCL1), but significantly triggered cell accumulation at the sub-G1 phase, loss of mitochondrial membrane potential, and expression of active caspase-3 and -8. In order to verify the effect of NF-ĸB inhibitor on expression of anti-apoptotic proteins, we performed western blotting. We found that the of NF-ĸB inhibitor or amentoflavone markedly diminished protein levels of MCL1 and C-FLIP. Taken all together, our findings show that amentoflavone induces intrinsic and extrinsic apoptosis and inhibits NF-ĸB-modulated anti-apoptotic signaling in U-87 MG cells in vitro.
原文英語
頁(從 - 到)279-285
頁數7
期刊In Vivo
32
發行號2
DOIs
出版狀態已發佈 - 二月 25 2018

指紋

Glioblastoma
Apoptosis
Myeloid Leukemia
Myeloid Cells
Fas-Associated Death Domain Protein
Caspase 1
Apoptosis Regulatory Proteins
Proteins
Caspase 8
Mitochondrial Membrane Potential
G1 Phase
Protein C
Interleukin-1beta
Caspase 3
Cell Survival
Western Blotting
Chemical activation
Cells
Membranes
diadenosine pyrophosphate

引用此文

Amentoflavone Induces Apoptosis and Inhibits NF-ĸB-modulated Anti-apoptotic Signaling in Glioblastoma Cells. / Yen, Tsung-Hsien; Hsieh, Chia-Ling; Liu, Tsu-Te; Huang, Chih-Sheng; Chen, Yen-Chung; Chuang, Yao-Chen; Lin, Song-Shei; Hsu, Fei-Ting.

於: In Vivo, 卷 32, 編號 2, 25.02.2018, p. 279-285.

研究成果: 雜誌貢獻文章

Yen, T-H, Hsieh, C-L, Liu, T-T, Huang, C-S, Chen, Y-C, Chuang, Y-C, Lin, S-S & Hsu, F-T 2018, 'Amentoflavone Induces Apoptosis and Inhibits NF-ĸB-modulated Anti-apoptotic Signaling in Glioblastoma Cells', In Vivo, 卷 32, 編號 2, 頁 279-285. https://doi.org/10.21873/invivo.11235
Yen, Tsung-Hsien ; Hsieh, Chia-Ling ; Liu, Tsu-Te ; Huang, Chih-Sheng ; Chen, Yen-Chung ; Chuang, Yao-Chen ; Lin, Song-Shei ; Hsu, Fei-Ting. / Amentoflavone Induces Apoptosis and Inhibits NF-ĸB-modulated Anti-apoptotic Signaling in Glioblastoma Cells. 於: In Vivo. 2018 ; 卷 32, 編號 2. 頁 279-285.
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abstract = "The goal of the present study was to investigate anticancer effect of amentoflavone on glioblastoma cells in vitro. Our results demonstrated that amentoflavone not only significantly reduced cell viability, nuclear factor-ĸappa B (NF-ĸB) activation, and protein expression of cellular Fas-associated protein with death domain-like interleukin 1 beta-converting enzyme inhibitory protein (C-FLIP) and myeloid cell leukemia 1 (MCL1), but significantly triggered cell accumulation at the sub-G1 phase, loss of mitochondrial membrane potential, and expression of active caspase-3 and -8. In order to verify the effect of NF-ĸB inhibitor on expression of anti-apoptotic proteins, we performed western blotting. We found that the of NF-ĸB inhibitor or amentoflavone markedly diminished protein levels of MCL1 and C-FLIP. Taken all together, our findings show that amentoflavone induces intrinsic and extrinsic apoptosis and inhibits NF-ĸB-modulated anti-apoptotic signaling in U-87 MG cells in vitro.",
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AU - Yen, Tsung-Hsien

AU - Hsieh, Chia-Ling

AU - Liu, Tsu-Te

AU - Huang, Chih-Sheng

AU - Chen, Yen-Chung

AU - Chuang, Yao-Chen

AU - Lin, Song-Shei

AU - Hsu, Fei-Ting

N1 - Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

PY - 2018/2/25

Y1 - 2018/2/25

N2 - The goal of the present study was to investigate anticancer effect of amentoflavone on glioblastoma cells in vitro. Our results demonstrated that amentoflavone not only significantly reduced cell viability, nuclear factor-ĸappa B (NF-ĸB) activation, and protein expression of cellular Fas-associated protein with death domain-like interleukin 1 beta-converting enzyme inhibitory protein (C-FLIP) and myeloid cell leukemia 1 (MCL1), but significantly triggered cell accumulation at the sub-G1 phase, loss of mitochondrial membrane potential, and expression of active caspase-3 and -8. In order to verify the effect of NF-ĸB inhibitor on expression of anti-apoptotic proteins, we performed western blotting. We found that the of NF-ĸB inhibitor or amentoflavone markedly diminished protein levels of MCL1 and C-FLIP. Taken all together, our findings show that amentoflavone induces intrinsic and extrinsic apoptosis and inhibits NF-ĸB-modulated anti-apoptotic signaling in U-87 MG cells in vitro.

AB - The goal of the present study was to investigate anticancer effect of amentoflavone on glioblastoma cells in vitro. Our results demonstrated that amentoflavone not only significantly reduced cell viability, nuclear factor-ĸappa B (NF-ĸB) activation, and protein expression of cellular Fas-associated protein with death domain-like interleukin 1 beta-converting enzyme inhibitory protein (C-FLIP) and myeloid cell leukemia 1 (MCL1), but significantly triggered cell accumulation at the sub-G1 phase, loss of mitochondrial membrane potential, and expression of active caspase-3 and -8. In order to verify the effect of NF-ĸB inhibitor on expression of anti-apoptotic proteins, we performed western blotting. We found that the of NF-ĸB inhibitor or amentoflavone markedly diminished protein levels of MCL1 and C-FLIP. Taken all together, our findings show that amentoflavone induces intrinsic and extrinsic apoptosis and inhibits NF-ĸB-modulated anti-apoptotic signaling in U-87 MG cells in vitro.

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