Amentoflavone enhances sorafenib-induced apoptosis through extrinsic and intrinsic pathways in sorafenib-resistant hepatocellular carcinoma SK-hep1 cells in vitro

Wei Lung Chen, Chia Ling Hsieh, Jiann Hwa Chen, Chih Sheng Huang, Wei Ting Chen, Yu Cheng Kuo, Cheng Yu Chen, Fei Ting Hsu

研究成果: 雜誌貢獻 › 文章

5 引文 (Scopus)

摘要

The present study aimed to evaluate the effects of amentoflavone on sorafenib-induced apoptosis in sorafenib-resistant hepatocellular carcinoma (HCC) cells. The sorafenib-resistant SK-Hep1 (SK-Hep1R) cell line was established for the present study. Initially, the differences in sorafenib-induced cytotoxicity and apoptosis between wild-type SK-Hep1 and SK-Hep1R cells were verified using the MTT assay and flow cytometry. The effects of amentoflavone on sorafenib-induced cytotoxicity and apoptosis were then investigated using MTT, flow cytometry, DNA gel electrophoresis and western blot analysis. The results demonstrated that cell viability of SK-Hep1R cells was increased compared with that of SK-Hep1 cells following treatment with different concentrations of sorafenib for 24 h. Apoptosis of SK-Hep1R cells was lower than that of SK-Hep1 cells following treatment with 20 µM sorafenib for 24 h. Amentoflavone alone did not inhibit cell viability but significantly triggered sorafenib-induced cytotoxicity and apoptosis in SK-Hep1R cells. Amentoflavone not only reversed sorafenib-induced anti-apoptotic protein levels but also enhanced sorafenib-induced pro-apoptotic protein expression in SK-Hep1R cells. In conclusion, amentoflavone may be used as a sorafenib sensitizer to enhance sorafenib-induced cytotoxicity and trigger sorafenib-induced apoptosis through extrinsic and intrinsic pathways in SK-Hep1R cells.
原文英語
頁（從 - 到）3229-3234
頁數6
期刊Oncology Letters
卷14
發行號3
DOIs
出版狀態已發佈 - 2017

指紋

Hepatocellular Carcinoma
Apoptosis
Apoptosis Regulatory Proteins
sorafenib
In Vitro Techniques
amentoflavone
Cell Survival
Flow Cytometry
Electrophoresis
Western Blotting
Gels
Cell Line
DNA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

引用此文

Amentoflavone enhances sorafenib-induced apoptosis through extrinsic and intrinsic pathways in sorafenib-resistant hepatocellular carcinoma SK-hep1 cells in vitro. / Chen, Wei Lung; Hsieh, Chia Ling; Chen, Jiann Hwa; Huang, Chih Sheng; Chen, Wei Ting; Kuo, Yu Cheng; Chen, Cheng Yu; Hsu, Fei Ting.

於: Oncology Letters, 卷 14, 編號 3, 2017, p. 3229-3234.

研究成果: 雜誌貢獻 › 文章

Chen, WL, Hsieh, CL, Chen, JH, Huang, CS, Chen, WT, Kuo, YC, Chen, CY & Hsu, FT 2017, 'Amentoflavone enhances sorafenib-induced apoptosis through extrinsic and intrinsic pathways in sorafenib-resistant hepatocellular carcinoma SK-hep1 cells in vitro', Oncology Letters, 卷 14, 編號 3, 頁 3229-3234. https://doi.org/10.3892/ol.2017.6540
Chen WL, Hsieh CL, Chen JH, Huang CS, Chen WT, Kuo YC 等. Amentoflavone enhances sorafenib-induced apoptosis through extrinsic and intrinsic pathways in sorafenib-resistant hepatocellular carcinoma SK-hep1 cells in vitro. Oncology Letters. 2017;14(3):3229-3234. https://doi.org/10.3892/ol.2017.6540
Chen, Wei Lung ; Hsieh, Chia Ling ; Chen, Jiann Hwa ; Huang, Chih Sheng ; Chen, Wei Ting ; Kuo, Yu Cheng ; Chen, Cheng Yu ; Hsu, Fei Ting. / Amentoflavone enhances sorafenib-induced apoptosis through extrinsic and intrinsic pathways in sorafenib-resistant hepatocellular carcinoma SK-hep1 cells in vitro. 於: Oncology Letters. 2017 ; 卷 14, 編號 3. 頁 3229-3234.
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abstract = "The present study aimed to evaluate the effects of amentoflavone on sorafenib-induced apoptosis in sorafenib-resistant hepatocellular carcinoma (HCC) cells. The sorafenib-resistant SK-Hep1 (SK-Hep1R) cell line was established for the present study. Initially, the differences in sorafenib-induced cytotoxicity and apoptosis between wild-type SK-Hep1 and SK-Hep1R cells were verified using the MTT assay and flow cytometry. The effects of amentoflavone on sorafenib-induced cytotoxicity and apoptosis were then investigated using MTT, flow cytometry, DNA gel electrophoresis and western blot analysis. The results demonstrated that cell viability of SK-Hep1R cells was increased compared with that of SK-Hep1 cells following treatment with different concentrations of sorafenib for 24 h. Apoptosis of SK-Hep1R cells was lower than that of SK-Hep1 cells following treatment with 20 µM sorafenib for 24 h. Amentoflavone alone did not inhibit cell viability but significantly triggered sorafenib-induced cytotoxicity and apoptosis in SK-Hep1R cells. Amentoflavone not only reversed sorafenib-induced anti-apoptotic protein levels but also enhanced sorafenib-induced pro-apoptotic protein expression in SK-Hep1R cells. In conclusion, amentoflavone may be used as a sorafenib sensitizer to enhance sorafenib-induced cytotoxicity and trigger sorafenib-induced apoptosis through extrinsic and intrinsic pathways in SK-Hep1R cells.",
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AU - Chen, Wei Lung

AU - Hsieh, Chia Ling

AU - Chen, Jiann Hwa

AU - Huang, Chih Sheng

AU - Chen, Wei Ting

AU - Kuo, Yu Cheng

AU - Chen, Cheng Yu

AU - Hsu, Fei Ting

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AB - The present study aimed to evaluate the effects of amentoflavone on sorafenib-induced apoptosis in sorafenib-resistant hepatocellular carcinoma (HCC) cells. The sorafenib-resistant SK-Hep1 (SK-Hep1R) cell line was established for the present study. Initially, the differences in sorafenib-induced cytotoxicity and apoptosis between wild-type SK-Hep1 and SK-Hep1R cells were verified using the MTT assay and flow cytometry. The effects of amentoflavone on sorafenib-induced cytotoxicity and apoptosis were then investigated using MTT, flow cytometry, DNA gel electrophoresis and western blot analysis. The results demonstrated that cell viability of SK-Hep1R cells was increased compared with that of SK-Hep1 cells following treatment with different concentrations of sorafenib for 24 h. Apoptosis of SK-Hep1R cells was lower than that of SK-Hep1 cells following treatment with 20 µM sorafenib for 24 h. Amentoflavone alone did not inhibit cell viability but significantly triggered sorafenib-induced cytotoxicity and apoptosis in SK-Hep1R cells. Amentoflavone not only reversed sorafenib-induced anti-apoptotic protein levels but also enhanced sorafenib-induced pro-apoptotic protein expression in SK-Hep1R cells. In conclusion, amentoflavone may be used as a sorafenib sensitizer to enhance sorafenib-induced cytotoxicity and trigger sorafenib-induced apoptosis through extrinsic and intrinsic pathways in SK-Hep1R cells.

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