Altering the Tat-derived peptide bioactivity landscape by changing the arginine side chain length

Cheng Hsun Wu, Yi Ping Chen, Chung Yuan Mou, Richard P. Cheng

研究成果: 雜誌貢獻文章同行評審

12 引文 斯高帕斯(Scopus)

摘要

Mutations of proteins with dual activities that lead to enhancement of one activity are frequently accompanied by attenuation of the other activity. However, this mutational negative trade-off phenomenon typically only involves the canonical 20 amino acids. To test the effect of non-canonical amino acids on the negative trade-off phenomenon, two bioactivities of HIV-1 Tat-derived peptides were monitored upon changing the Arg side chain length. In contrast to the expected mutational negative trade-off, shortening Arg by one methylene resulted in both higher TAR RNA binding specificity and higher cellular uptake. These results suggest that introducing previously unexploited building blocks, even if the difference is only one methylene, can alter the peptide bioactivity landscape leading to the enhancement of multiple bioactivities.

原文英語
頁(從 - 到)473-480
頁數8
期刊Amino Acids
44
發行號2
DOIs
出版狀態已發佈 - 二月 2013
對外發佈

ASJC Scopus subject areas

  • 生物化學
  • 臨床生物化學
  • 有機化學

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