Altering the Tat-derived peptide bioactivity landscape by changing the arginine side chain length

Cheng Hsun Wu, Yi Ping Chen, Chung Yuan Mou, Richard P. Cheng

研究成果: 雜誌貢獻文章

11 引文 (Scopus)

摘要

Mutations of proteins with dual activities that lead to enhancement of one activity are frequently accompanied by attenuation of the other activity. However, this mutational negative trade-off phenomenon typically only involves the canonical 20 amino acids. To test the effect of non-canonical amino acids on the negative trade-off phenomenon, two bioactivities of HIV-1 Tat-derived peptides were monitored upon changing the Arg side chain length. In contrast to the expected mutational negative trade-off, shortening Arg by one methylene resulted in both higher TAR RNA binding specificity and higher cellular uptake. These results suggest that introducing previously unexploited building blocks, even if the difference is only one methylene, can alter the peptide bioactivity landscape leading to the enhancement of multiple bioactivities.

原文英語
頁(從 - 到)473-480
頁數8
期刊Amino Acids
44
發行號2
DOIs
出版狀態已發佈 - 二月 2013
對外發佈Yes

指紋

Bioactivity
Chain length
Arginine
Amino Acids
Peptides
HIV-1
RNA
Mutation
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

引用此文

Altering the Tat-derived peptide bioactivity landscape by changing the arginine side chain length. / Wu, Cheng Hsun; Chen, Yi Ping; Mou, Chung Yuan; Cheng, Richard P.

於: Amino Acids, 卷 44, 編號 2, 02.2013, p. 473-480.

研究成果: 雜誌貢獻文章

Wu, Cheng Hsun ; Chen, Yi Ping ; Mou, Chung Yuan ; Cheng, Richard P. / Altering the Tat-derived peptide bioactivity landscape by changing the arginine side chain length. 於: Amino Acids. 2013 ; 卷 44, 編號 2. 頁 473-480.
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