Altered prostate epithelial development and IGF-1 signal in mice lacking the androgen receptor in stromal smooth muscle cells

Shengqiang Yu, Caixia Zhang, Chiu Chun Lin, Yuanjie Niu, Kuo Pao Lai, Hong Chiang Chang, Shauh Der Yeh, Chawnshang Chang, Shuyuan Yeh

研究成果: 雜誌貢獻文章同行評審

38 引文 斯高帕斯(Scopus)

摘要

Background Androgens and the androgen receptor (AR) play critical roles in the prostate development via mesenchymal-epithelial interactions. Smooth muscle cells (SMC), differentiated from mesenchyme, are one of the basic components of the prostate stroma. However, the roles of smooth muscle AR in prostate development are still obscure. Methods We established the smooth muscle selective AR knockout (SM-ARKO) mouse model using the Cre-loxP system, and confirmed the ARKO efficiency at RNA, DNA and protein levels. Then, we observed the prostate morphology changes, and determined the epithelial proliferation, apoptosis, and differentiation. We also knocked down the AR in a prostate smooth muscle cell line (PS-1) to confirm the in vivo findings and to probe the mechanism. Results The AR was selectively and efficiently knocked out in the anterior prostates of SM-ARKO mouse. The SM-ARKO prostates have defects with loss of infolding structures, and decrease of epithelial proliferation, but with little change of apoptosis and differentiation. The mechanism studies showed that IGF-1 expression level decreased in the SM-ARKO prostates and AR-knockdown PS-1 cells. The decreased IGF-1 expression might contribute to the defective development of SM-ARKO prostates. ConclusionS The AR in SMCs plays important roles in the prostate development via the regulation of IGF-1 signal. Prostate 77:517-524, 2011.
原文英語
頁(從 - 到)517-524
頁數8
期刊Prostate
71
發行號5
DOIs
出版狀態已發佈 - 四月 2011

ASJC Scopus subject areas

  • 腫瘤科
  • 泌尿科學

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