Altered perlecan expression in placental development and gestational diabetes mellitus

W. C V Yang, T. H. Su, Y. C. Yang, S. C. Chang, C. Y. Chen, C. P. Chen

研究成果: 雜誌貢獻文章

18 引文 (Scopus)

摘要

The proteoglycan perlecan is involved in cell signaling, regulation of growth factor activity, and maintenance of basement membranes. This study aims to investigate the expression of perlecan during placental development and whether hyperglycemia of gestational diabetes mellitus induces the alteration of perlecan expression in placenta. Immunohistochemistry, immunoprecipitation/sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and quantitative real-time PCR were carried out to study the placental perlecan expression at different trimesters of pregnancies and in gestational diabetes mellitus. The perlecan protein was mainly immunolocalized in the trophoblast and vessel basement membranes with some staining in the villous stroma of placental villus. Perlecan was also found to co-localize with laminin and collagen IV in the basement membranes of placenta. The protein and mRNA levels of placental perlecan were significantly decreased as the gestational age increased. However, a significant increase in perlecan expression was observed in the third trimester placentas with gestational diabetes mellitus compared to the gestational age-matched controls. Furthermore, trophoblast cells cultured in a high glucose (30 mM) medium and a high osmotic pressure medium (5.6 mM glucose and 24.4 mM mannitol) showed increased perlecan expression compared to cells cultured in the low glucose (5.6 mM) regular medium. These alterations of perlecan expression may be associated with the structural changes of placenta during maturation. The metabolic effect of high glucose and high osmotic pressure of gestational diabetes mellitus may contribute to the increased perlecan expression of diabetic placentas.
原文英語
頁(從 - 到)780-788
頁數9
期刊Placenta
26
發行號10
DOIs
出版狀態已發佈 - 十一月 2005

指紋

Placentation
Gestational Diabetes
Placenta
Basement Membrane
Glucose
Osmotic Pressure
Trophoblasts
Gestational Age
Cultured Cells
perlecan
Pregnancy in Diabetics
Chorionic Villi
Third Pregnancy Trimester
Mannitol
Laminin
Proteoglycans
Immunoprecipitation
Sodium Dodecyl Sulfate
Hyperglycemia
Real-Time Polymerase Chain Reaction

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

引用此文

Altered perlecan expression in placental development and gestational diabetes mellitus. / Yang, W. C V; Su, T. H.; Yang, Y. C.; Chang, S. C.; Chen, C. Y.; Chen, C. P.

於: Placenta, 卷 26, 編號 10, 11.2005, p. 780-788.

研究成果: 雜誌貢獻文章

Yang, W. C V ; Su, T. H. ; Yang, Y. C. ; Chang, S. C. ; Chen, C. Y. ; Chen, C. P. / Altered perlecan expression in placental development and gestational diabetes mellitus. 於: Placenta. 2005 ; 卷 26, 編號 10. 頁 780-788.
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