Altered pattern of monocyte differentiation and monocyte-derived TGF-β1 in severe asthma

Chih Hsing Hung, Chin Chou Wang, Jau Ling Suen, Chau Chyun Sheu, Chang Hung Kuo, Wei Ting Liao, Yi Hsin Yang, Chao Chien Wu, Sum Yee Leung, Ruay Sheng Lai, Chi Cheng Lin, Yu Feng Wei, Chong Yeh Lee, Ming Shyan Huang, Shau Ku Huang

研究成果: 雜誌貢獻文章

9 引文 斯高帕斯(Scopus)

摘要

CD14+ monocytes contain precursors for macrophages and fibrocytes, known to be involved in regulating airway remodeling in human asthma and distinguishable by the PM-2K marker. We sought to identify circulating subsets of PM-2K+ macrophage-like cells and evaluate their relationships to lung function, severity and control status. Circulating PM-2K+ macrophage-like cells and fibrocytes could be identified and distinguished between normal individuals (N = 152) and asthmatic subjects (N = 133) using multi-parametric flow cytometry. PM-2K+ macrophage-like cells were found to be significantly lower in asthmatic subjects, particularly noted for the CD14-PM-2K+ subset and PM-2K+CCR7-CD86+ cells in subjects with poor lung function (FEV%/FVC% < 80%) as compared to those of normal subjects and asthmatics with normal lung function, whereas the frequency of fibrocytes was higher in asthmatics and the CCR7-CD86+ subset distribution was significantly different in subjects with varying severity. Moreover, exogenous transforming growth factor beta 1 (TGF-β1) was found to inhibit the generation of PM-2K+ macrophage-like cells, but promote the growth of fibrocytes, from CD14+ monocytes, and monocyte-derived TGF-β1 was found to correlate with the lung function, severity and control status in asthmatic patients. Collectively, aberrant differentiation of monocytes into PM-2K+ macrophage-like cell subsets and fibrocytes, together with increased monocyte-derived TGF-β1, characterized patients with severe asthma.

原文英語
文章編號919
期刊Scientific Reports
8
發行號1
DOIs
出版狀態已發佈 - 十二月 1 2018
對外發佈Yes

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    Hung, C. H., Wang, C. C., Suen, J. L., Sheu, C. C., Kuo, C. H., Liao, W. T., Yang, Y. H., Wu, C. C., Leung, S. Y., Lai, R. S., Lin, C. C., Wei, Y. F., Lee, C. Y., Huang, M. S., & Huang, S. K. (2018). Altered pattern of monocyte differentiation and monocyte-derived TGF-β1 in severe asthma. Scientific Reports, 8(1), [919]. https://doi.org/10.1038/s41598-017-19105-z