摘要

Mesenchymal stem cell (MSC) is mechanosensitive and the respond to mechanical force is pattern specific. We previously reported that oscillatory shear stress at 0.5 ± 4 dyne/cm2 guided MSCs polarity vertical to net flow direction before apolaric stage at 30 min resulting in phosphorylation of β-catenin and inhibition of Wnt signaling. This time, we explored laminar shear stress (LS) at 0.5 dyne/cm2 polarized MSCs by guiding F-actin orientation parallel to the flow direction before apolarity at 30 min accompanied with activation of Wnt signaling. Time-dependent microarray analysis supported cell-cell junctional complex of MSCs was the major mechanosensor on MSCs to respond 0.5 dyne/cm2 LS. Three-dimensional immunofluorescence image confirmed LS promoting β-catenin nuclear localization during 15 min to 1 h with a peak at 30 min. Functional analysis of proteomic study on MSC with 30 min LS stimulation indicated that upregulation of β-catenin downstream proteins related to cardiovascular development, endothelial cell protection and angiogenesis. Conditioned medium from MSCs with 30 min LS stimulation improved the viability of human endothelial cells from oxidative damage. In conclusion, 0.5 dyne/cm2 LS on MSCs for 30 min guides MSCs lack of polarity and promotes β-catenin nuclear translocation favoring Wnt activation and paracrine cardiovascular support.
原文英語
頁(從 - 到)1-10
頁數10
期刊Biomaterials
190-191
DOIs
出版狀態已發佈 - 一月 1 2019

指紋

Catenins
Cell Polarity
Stem cells
Mesenchymal Stromal Cells
Shear stress
Endothelial Cells
Tissue Array Analysis
Endothelial cells
Cytoprotection
Three-Dimensional Imaging
Conditioned Culture Medium
Proteomics
Fluorescent Antibody Technique
Actins
Chemical activation
Up-Regulation
Phosphorylation
Functional analysis
Microarrays
Proteins

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

引用此文

Alteration of mesenchymal stem cells polarity by laminar shear stimulation promoting β-catenin nuclear localization. / Chen, Wei-Ta; Hsu, Wei-Tse; Yen, Meng-Hua; Changou, Chun A; Han, Chia-Li; Chen, Yu-Ju; Cheng, Ji-Yen; Chang, Tzu-Hao; Lee, Oscar Kuang-Sheng; Ho, Jennifer Hui-Chun.

於: Biomaterials, 卷 190-191, 01.01.2019, p. 1-10.

研究成果: 雜誌貢獻文章

Chen, Wei-Ta ; Hsu, Wei-Tse ; Yen, Meng-Hua ; Changou, Chun A ; Han, Chia-Li ; Chen, Yu-Ju ; Cheng, Ji-Yen ; Chang, Tzu-Hao ; Lee, Oscar Kuang-Sheng ; Ho, Jennifer Hui-Chun. / Alteration of mesenchymal stem cells polarity by laminar shear stimulation promoting β-catenin nuclear localization. 於: Biomaterials. 2019 ; 卷 190-191. 頁 1-10.
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abstract = "Mesenchymal stem cell (MSC) is mechanosensitive and the respond to mechanical force is pattern specific. We previously reported that oscillatory shear stress at 0.5 ± 4 dyne/cm2 guided MSCs polarity vertical to net flow direction before apolaric stage at 30 min resulting in phosphorylation of β-catenin and inhibition of Wnt signaling. This time, we explored laminar shear stress (LS) at 0.5 dyne/cm2 polarized MSCs by guiding F-actin orientation parallel to the flow direction before apolarity at 30 min accompanied with activation of Wnt signaling. Time-dependent microarray analysis supported cell-cell junctional complex of MSCs was the major mechanosensor on MSCs to respond 0.5 dyne/cm2 LS. Three-dimensional immunofluorescence image confirmed LS promoting β-catenin nuclear localization during 15 min to 1 h with a peak at 30 min. Functional analysis of proteomic study on MSC with 30 min LS stimulation indicated that upregulation of β-catenin downstream proteins related to cardiovascular development, endothelial cell protection and angiogenesis. Conditioned medium from MSCs with 30 min LS stimulation improved the viability of human endothelial cells from oxidative damage. In conclusion, 0.5 dyne/cm2 LS on MSCs for 30 min guides MSCs lack of polarity and promotes β-catenin nuclear translocation favoring Wnt activation and paracrine cardiovascular support.",
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author = "Wei-Ta Chen and Wei-Tse Hsu and Meng-Hua Yen and Changou, {Chun A} and Chia-Li Han and Yu-Ju Chen and Ji-Yen Cheng and Tzu-Hao Chang and Lee, {Oscar Kuang-Sheng} and Ho, {Jennifer Hui-Chun}",
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AU - Chen, Wei-Ta

AU - Hsu, Wei-Tse

AU - Yen, Meng-Hua

AU - Changou, Chun A

AU - Han, Chia-Li

AU - Chen, Yu-Ju

AU - Cheng, Ji-Yen

AU - Chang, Tzu-Hao

AU - Lee, Oscar Kuang-Sheng

AU - Ho, Jennifer Hui-Chun

N1 - Copyright © 2018. Published by Elsevier Ltd.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Mesenchymal stem cell (MSC) is mechanosensitive and the respond to mechanical force is pattern specific. We previously reported that oscillatory shear stress at 0.5 ± 4 dyne/cm2 guided MSCs polarity vertical to net flow direction before apolaric stage at 30 min resulting in phosphorylation of β-catenin and inhibition of Wnt signaling. This time, we explored laminar shear stress (LS) at 0.5 dyne/cm2 polarized MSCs by guiding F-actin orientation parallel to the flow direction before apolarity at 30 min accompanied with activation of Wnt signaling. Time-dependent microarray analysis supported cell-cell junctional complex of MSCs was the major mechanosensor on MSCs to respond 0.5 dyne/cm2 LS. Three-dimensional immunofluorescence image confirmed LS promoting β-catenin nuclear localization during 15 min to 1 h with a peak at 30 min. Functional analysis of proteomic study on MSC with 30 min LS stimulation indicated that upregulation of β-catenin downstream proteins related to cardiovascular development, endothelial cell protection and angiogenesis. Conditioned medium from MSCs with 30 min LS stimulation improved the viability of human endothelial cells from oxidative damage. In conclusion, 0.5 dyne/cm2 LS on MSCs for 30 min guides MSCs lack of polarity and promotes β-catenin nuclear translocation favoring Wnt activation and paracrine cardiovascular support.

AB - Mesenchymal stem cell (MSC) is mechanosensitive and the respond to mechanical force is pattern specific. We previously reported that oscillatory shear stress at 0.5 ± 4 dyne/cm2 guided MSCs polarity vertical to net flow direction before apolaric stage at 30 min resulting in phosphorylation of β-catenin and inhibition of Wnt signaling. This time, we explored laminar shear stress (LS) at 0.5 dyne/cm2 polarized MSCs by guiding F-actin orientation parallel to the flow direction before apolarity at 30 min accompanied with activation of Wnt signaling. Time-dependent microarray analysis supported cell-cell junctional complex of MSCs was the major mechanosensor on MSCs to respond 0.5 dyne/cm2 LS. Three-dimensional immunofluorescence image confirmed LS promoting β-catenin nuclear localization during 15 min to 1 h with a peak at 30 min. Functional analysis of proteomic study on MSC with 30 min LS stimulation indicated that upregulation of β-catenin downstream proteins related to cardiovascular development, endothelial cell protection and angiogenesis. Conditioned medium from MSCs with 30 min LS stimulation improved the viability of human endothelial cells from oxidative damage. In conclusion, 0.5 dyne/cm2 LS on MSCs for 30 min guides MSCs lack of polarity and promotes β-catenin nuclear translocation favoring Wnt activation and paracrine cardiovascular support.

KW - Cardiovascular

KW - Laminar shear

KW - Mesenchymal stem cells

KW - Wnt

KW - β-catenin

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