Alpinate oxyphyllae fructus (Alpinia oxyphylla miq) extracts inhibit angiotensin-II induced cardiac apoptosis in H9c2 cardiomyoblast cells

Yung Ming Chang, Chuan Te Tsai, Chiun Chuang Roger Wang, Yueh Sheng Chen, Yueh Min Lin, Chia Hua Kuo, Bor Show Tzang, Ray Jade Chen, Fuu Jen Tsai, Chih Yang Huang

研究成果: 雜誌貢獻文章同行評審

15 引文 斯高帕斯(Scopus)

摘要

Angiotensin II (Ang II) is a risk factor for cardiovascular disease. We used a traditional Chinese medicine, alpinate oxyphyllae fructus (AOF), to evaluate its effect on Ang II-induced cardiac apoptosis and mitochondrial dysfunction. Ang II-treated H9c2 cells were administered AOF of 20-100μg/mL concentrations. Ang II significantly increased TUNEL-positive nuclei in the H9c2 cells, effect was inhibited by AOF administration in both pre-treated and post-treated H9c2 cells. Caspases 9 and 3 activities were increased by Ang II and downregulated by AOF administration, especially in pre-treatment. AOF treatment reversed Ang II-induced mitochondria membrane potential instability in H9c2 cells as observed by JC-1 stain assay. Furthermore, proapoptotic proteins Bad and cytochrome c increased and decreased respectively under AOF administration. The levels of p-Bad anti-apoptotic protein were significantly increased after AOF treatment. This study indicates that mitochondrial dependent apoptosis induced by Ang II.

原文英語
頁(從 - 到)229-234
頁數6
期刊Bioscience, Biotechnology and Biochemistry
77
發行號2
DOIs
出版狀態已發佈 - 2013

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

指紋 深入研究「Alpinate oxyphyllae fructus (Alpinia oxyphylla miq) extracts inhibit angiotensin-II induced cardiac apoptosis in H9c2 cardiomyoblast cells」主題。共同形成了獨特的指紋。

引用此