Alginate/poly(γ-glutamic acid) base biocompatible gel for bone tissue engineering

Wing P. Chan, Fu Chen Kung, Yu Lin Kuo, Ming Chen Yang, Wen-FuThomas Lai

研究成果: 雜誌貢獻文章

9 引文 (Scopus)

摘要

A technique for synthesizing biocompatible hydrogels by cross-linking calcium-form poly(γ-glutamic acid), alginate sodium, and Pluronic F-127 was created, in which alginate can be cross-linked by Ca2+ from Ca-γ-PGA directly and γ-PGA molecules introduced into the alginate matrix to provide pH sensitivity and hemostasis. Mechanical properties, swelling behavior, and blood compatibility were investigated for each hydrogel compared with alginate and for γ-PGA hydrogel with the sodium form only. Adding F-127 improves mechanical properties efficiently and influences the temperature-sensitive swelling of the hydrogels but also has a minor effect on pH-sensitive swelling and promotes anticoagulation. MG-63 cells were used to test biocompatibility. Gelation occurred gradually through change in the elastic modulus as the release of calcium ions increased over time and caused ionic cross-linking, which promotes the elasticity of gel. In addition, the growth of MG-63 cells in the gel reflected nontoxicity. These results showed that this biocompatible scaffold has potential for application in bone materials.
原文英語
文章編號185841
期刊BioMed Research International
2015
DOIs
出版狀態已發佈 - 2015

指紋

Tissue Engineering
Prostaglandins A
Tissue engineering
Glutamic Acid
Bone
Gels
Swelling
Bone and Bones
Hydrogels
Hydrogel
Calcium
Poloxamer
Mechanical properties
Elastic Modulus
Elasticity
Gelation
Hemostasis
Biocompatibility
Scaffolds
Blood

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

引用此文

Alginate/poly(γ-glutamic acid) base biocompatible gel for bone tissue engineering. / Chan, Wing P.; Kung, Fu Chen; Kuo, Yu Lin; Yang, Ming Chen; Lai, Wen-FuThomas.

於: BioMed Research International, 卷 2015, 185841, 2015.

研究成果: 雜誌貢獻文章

Chan, Wing P. ; Kung, Fu Chen ; Kuo, Yu Lin ; Yang, Ming Chen ; Lai, Wen-FuThomas. / Alginate/poly(γ-glutamic acid) base biocompatible gel for bone tissue engineering. 於: BioMed Research International. 2015 ; 卷 2015.
@article{df4a2e558b574364b2470d4e57acea0b,
title = "Alginate/poly(γ-glutamic acid) base biocompatible gel for bone tissue engineering",
abstract = "A technique for synthesizing biocompatible hydrogels by cross-linking calcium-form poly(γ-glutamic acid), alginate sodium, and Pluronic F-127 was created, in which alginate can be cross-linked by Ca2+ from Ca-γ-PGA directly and γ-PGA molecules introduced into the alginate matrix to provide pH sensitivity and hemostasis. Mechanical properties, swelling behavior, and blood compatibility were investigated for each hydrogel compared with alginate and for γ-PGA hydrogel with the sodium form only. Adding F-127 improves mechanical properties efficiently and influences the temperature-sensitive swelling of the hydrogels but also has a minor effect on pH-sensitive swelling and promotes anticoagulation. MG-63 cells were used to test biocompatibility. Gelation occurred gradually through change in the elastic modulus as the release of calcium ions increased over time and caused ionic cross-linking, which promotes the elasticity of gel. In addition, the growth of MG-63 cells in the gel reflected nontoxicity. These results showed that this biocompatible scaffold has potential for application in bone materials.",
author = "Chan, {Wing P.} and Kung, {Fu Chen} and Kuo, {Yu Lin} and Yang, {Ming Chen} and Wen-FuThomas Lai",
year = "2015",
doi = "10.1155/2015/185841",
language = "English",
volume = "2015",
journal = "BioMed Research International",
issn = "2314-6133",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Alginate/poly(γ-glutamic acid) base biocompatible gel for bone tissue engineering

AU - Chan, Wing P.

AU - Kung, Fu Chen

AU - Kuo, Yu Lin

AU - Yang, Ming Chen

AU - Lai, Wen-FuThomas

PY - 2015

Y1 - 2015

N2 - A technique for synthesizing biocompatible hydrogels by cross-linking calcium-form poly(γ-glutamic acid), alginate sodium, and Pluronic F-127 was created, in which alginate can be cross-linked by Ca2+ from Ca-γ-PGA directly and γ-PGA molecules introduced into the alginate matrix to provide pH sensitivity and hemostasis. Mechanical properties, swelling behavior, and blood compatibility were investigated for each hydrogel compared with alginate and for γ-PGA hydrogel with the sodium form only. Adding F-127 improves mechanical properties efficiently and influences the temperature-sensitive swelling of the hydrogels but also has a minor effect on pH-sensitive swelling and promotes anticoagulation. MG-63 cells were used to test biocompatibility. Gelation occurred gradually through change in the elastic modulus as the release of calcium ions increased over time and caused ionic cross-linking, which promotes the elasticity of gel. In addition, the growth of MG-63 cells in the gel reflected nontoxicity. These results showed that this biocompatible scaffold has potential for application in bone materials.

AB - A technique for synthesizing biocompatible hydrogels by cross-linking calcium-form poly(γ-glutamic acid), alginate sodium, and Pluronic F-127 was created, in which alginate can be cross-linked by Ca2+ from Ca-γ-PGA directly and γ-PGA molecules introduced into the alginate matrix to provide pH sensitivity and hemostasis. Mechanical properties, swelling behavior, and blood compatibility were investigated for each hydrogel compared with alginate and for γ-PGA hydrogel with the sodium form only. Adding F-127 improves mechanical properties efficiently and influences the temperature-sensitive swelling of the hydrogels but also has a minor effect on pH-sensitive swelling and promotes anticoagulation. MG-63 cells were used to test biocompatibility. Gelation occurred gradually through change in the elastic modulus as the release of calcium ions increased over time and caused ionic cross-linking, which promotes the elasticity of gel. In addition, the growth of MG-63 cells in the gel reflected nontoxicity. These results showed that this biocompatible scaffold has potential for application in bone materials.

UR - http://www.scopus.com/inward/record.url?scp=84945319702&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84945319702&partnerID=8YFLogxK

U2 - 10.1155/2015/185841

DO - 10.1155/2015/185841

M3 - Article

C2 - 26504784

AN - SCOPUS:84945319702

VL - 2015

JO - BioMed Research International

JF - BioMed Research International

SN - 2314-6133

M1 - 185841

ER -