Aglycone geniposidic acid, a naturally occurring crosslinking agent, and its application for the fixation of collagenous tissues

Fwu Long Mi, Chin Tsung Huang, Ya Ling Chiu, Mei Chin Chen, Hsiang F. Liang, Hsing W. Sung

研究成果: 雜誌貢獻文章

3 引文 斯高帕斯(Scopus)

摘要

A natural compound, aglycone geniposidic acid (aGSA), originated from the fruits of Gardenia jasminoides ELLIS was used for the fixation of collagenous tissues. The presumed crosslinking reaction mechanism of collagenous tissues with aGSA was inferred by reacting aGSA with a bifunctional amine, 1,6-hexanediamine, using a series of 1H NMR, FT-IR, and UV/Vis spectra analyses. aGSA reacted with 1,6-hexanediamine by a nucleophilic attack on the olefinic carbon atom at C-2 of deoxyloganin aglycone, followed by opening the dihydropyran ring to form heterocyclic amine compounds. It is inferred that aGSA may form intramolecular and intermolecular crosslinks with a heterocyclic structure within collagen fibers in tissues. The degrees of tissue fixation by aGSA at different pH values were investigated by examining the fixation indices and denaturation temperatures of test samples. It was found that the fixation indices and denaturation temperatures of test samples fixed at neutral or basic pH (pH 7.4 or pH 8.5) were significantly greater than at acidic pH (pH 4.0). The results obtained in this study may be used to elucidate the crosslinking mechanism and optimize the fixation process for developing bioprostheses fixed by aGSA.

原文英語
頁(從 - 到)667-673
頁數7
期刊Journal of Biomedical Materials Research - Part A
83
發行號3
DOIs
出版狀態已發佈 - 十一月 1 2007
對外發佈Yes

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

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