Affinity chromatography in the industrial purification of plasma proteins for therapeutic use

Thierry Burnouf, Mirjana Radosevich

研究成果: 雜誌貢獻回顧型文獻

79 引文 (Scopus)

摘要

Affinity chromatography is a powerful technique for the purification of many proteins in human plasma. Applications cover the isolation of proteins for research purposes but also, to a large extent, for the production of therapeutic products. In industrial plasma fractionation, affinity chromatography has been found to be particularly advantageous for fine and rapid capture of plasma proteins from industrial plasma fractions pre-purified by ethanol fractionation or by ion-exchange chromatography. To date, affinity chromatography is being used in the production of various licensed therapeutic plasma products, such as the concentrates of Factor VIII, Factor IX, von Willebrand Factor, Protein C, Antithrombin III, and Factor XI. Most commonly used ligands are heparin, gelatin, murine antibodies, and, to a lesser extent, Cu2+. Possible development of the use of affinity chromatography in industrial plasma fractionation should be associated to the current development of phage display and combinatorial chemistry. Both approaches may lead to the development of tailor-made synthetic ligands that would allow implementation of protein capture technology, providing improved productivity and yield for plasma products.
原文英語
頁(從 - 到)575-586
頁數12
期刊Journal of Biochemical and Biophysical Methods
49
發行號1-3
DOIs
出版狀態已發佈 - 十月 30 2001
對外發佈Yes

指紋

Affinity chromatography
Therapeutic Uses
Affinity Chromatography
Purification
Blood Proteins
Plasmas
Fractionation
von Willebrand Factor
Factor XI
Ligands
Plasma (human)
Proteins
Factor IX
Bacteriophages
Antithrombin III
Factor VIII
Gelatin
Chromatography
Protein C
Ion Exchange Chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

引用此文

Affinity chromatography in the industrial purification of plasma proteins for therapeutic use. / Burnouf, Thierry; Radosevich, Mirjana.

於: Journal of Biochemical and Biophysical Methods, 卷 49, 編號 1-3, 30.10.2001, p. 575-586.

研究成果: 雜誌貢獻回顧型文獻

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