Advanced glycosylated end products restrain the osteogenic differentiation of the periodontal ligament stem cell

Zhu Ling Guo, Shan Ling Gan, Chun Yi Cao, Rao Fu, Sheng Ping Cao, Chen Xie, Jing Wei Chen, Alex Gibson, Xu Zheng, Nai chia Teng

研究成果: 雜誌貢獻文章

摘要

Background/purpose: Many studies have confirmed that periodontal disease interacts with diabetes. The aim of this study was to examine whether the advanced glycosylated end products (AGEs), which are generated by diabetics, have important effects on the osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Materials and methods: In this study PDLSCs were isolated from the periodontal ligaments of extracted third molar teeth. The subjects were divided into two groups, which included the normal control group (N-PDLSCs) and the AGEs-stimulating group (A-PDLSCs). Changes of receptor of AGEs (RAGE) and cumulative ROS in PDLSCs were monitored by western blot and flow cytometry, respectively. Results: In the study AGEs noticeably inhibited the osteogenic differentiation of PDLSCs, with significant lower calcification nodules detected in A-PDLSCs (P < 0.01). RAGE expression level and ROS accumulation in A-PDLSCs were clearly higher than those in N-PDLSCs (P < 0.01). Conclusion: Our conclusions were that AGEs may cause the apoptosis of stem cells, which could lead to the disorder of bone differentiation function of PDLSCs.
原文英語
期刊Journal of Dental Sciences
DOIs
出版狀態已發佈 - 一月 1 2019

指紋

Periodontal Ligament
Stem Cells
Third Molar
Periodontal Diseases
Tooth
Flow Cytometry
Western Blotting
Apoptosis

ASJC Scopus subject areas

  • Dentistry(all)

引用此文

Advanced glycosylated end products restrain the osteogenic differentiation of the periodontal ligament stem cell. / Guo, Zhu Ling; Gan, Shan Ling; Cao, Chun Yi; Fu, Rao; Cao, Sheng Ping; Xie, Chen; Chen, Jing Wei; Gibson, Alex; Zheng, Xu; Teng, Nai chia.

於: Journal of Dental Sciences, 01.01.2019.

研究成果: 雜誌貢獻文章

Guo, Zhu Ling ; Gan, Shan Ling ; Cao, Chun Yi ; Fu, Rao ; Cao, Sheng Ping ; Xie, Chen ; Chen, Jing Wei ; Gibson, Alex ; Zheng, Xu ; Teng, Nai chia. / Advanced glycosylated end products restrain the osteogenic differentiation of the periodontal ligament stem cell. 於: Journal of Dental Sciences. 2019.
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abstract = "Background/purpose: Many studies have confirmed that periodontal disease interacts with diabetes. The aim of this study was to examine whether the advanced glycosylated end products (AGEs), which are generated by diabetics, have important effects on the osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Materials and methods: In this study PDLSCs were isolated from the periodontal ligaments of extracted third molar teeth. The subjects were divided into two groups, which included the normal control group (N-PDLSCs) and the AGEs-stimulating group (A-PDLSCs). Changes of receptor of AGEs (RAGE) and cumulative ROS in PDLSCs were monitored by western blot and flow cytometry, respectively. Results: In the study AGEs noticeably inhibited the osteogenic differentiation of PDLSCs, with significant lower calcification nodules detected in A-PDLSCs (P < 0.01). RAGE expression level and ROS accumulation in A-PDLSCs were clearly higher than those in N-PDLSCs (P < 0.01). Conclusion: Our conclusions were that AGEs may cause the apoptosis of stem cells, which could lead to the disorder of bone differentiation function of PDLSCs.",
keywords = "AGEs (advanced glycosylated end products), Diabetes mellitus, PDLSCs (periodontal ligament stem cells), Periodontal disease, Prevention",
author = "Guo, {Zhu Ling} and Gan, {Shan Ling} and Cao, {Chun Yi} and Rao Fu and Cao, {Sheng Ping} and Chen Xie and Chen, {Jing Wei} and Alex Gibson and Xu Zheng and Teng, {Nai chia}",
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AU - Guo, Zhu Ling

AU - Gan, Shan Ling

AU - Cao, Chun Yi

AU - Fu, Rao

AU - Cao, Sheng Ping

AU - Xie, Chen

AU - Chen, Jing Wei

AU - Gibson, Alex

AU - Zheng, Xu

AU - Teng, Nai chia

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N2 - Background/purpose: Many studies have confirmed that periodontal disease interacts with diabetes. The aim of this study was to examine whether the advanced glycosylated end products (AGEs), which are generated by diabetics, have important effects on the osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Materials and methods: In this study PDLSCs were isolated from the periodontal ligaments of extracted third molar teeth. The subjects were divided into two groups, which included the normal control group (N-PDLSCs) and the AGEs-stimulating group (A-PDLSCs). Changes of receptor of AGEs (RAGE) and cumulative ROS in PDLSCs were monitored by western blot and flow cytometry, respectively. Results: In the study AGEs noticeably inhibited the osteogenic differentiation of PDLSCs, with significant lower calcification nodules detected in A-PDLSCs (P < 0.01). RAGE expression level and ROS accumulation in A-PDLSCs were clearly higher than those in N-PDLSCs (P < 0.01). Conclusion: Our conclusions were that AGEs may cause the apoptosis of stem cells, which could lead to the disorder of bone differentiation function of PDLSCs.

AB - Background/purpose: Many studies have confirmed that periodontal disease interacts with diabetes. The aim of this study was to examine whether the advanced glycosylated end products (AGEs), which are generated by diabetics, have important effects on the osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Materials and methods: In this study PDLSCs were isolated from the periodontal ligaments of extracted third molar teeth. The subjects were divided into two groups, which included the normal control group (N-PDLSCs) and the AGEs-stimulating group (A-PDLSCs). Changes of receptor of AGEs (RAGE) and cumulative ROS in PDLSCs were monitored by western blot and flow cytometry, respectively. Results: In the study AGEs noticeably inhibited the osteogenic differentiation of PDLSCs, with significant lower calcification nodules detected in A-PDLSCs (P < 0.01). RAGE expression level and ROS accumulation in A-PDLSCs were clearly higher than those in N-PDLSCs (P < 0.01). Conclusion: Our conclusions were that AGEs may cause the apoptosis of stem cells, which could lead to the disorder of bone differentiation function of PDLSCs.

KW - AGEs (advanced glycosylated end products)

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KW - PDLSCs (periodontal ligament stem cells)

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KW - Prevention

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