Adrenaline inhibits lipopolysaccharide-induced macrophage inflammatory protein-1α in human monocytes: The role of β-adrenergic receptors

Chi Yuan Li, Tz Chong Chou, Chian Her Lee, Chien Sung Tsai, Shih Hurng Loh, Chih Shung Wong

研究成果: 雜誌貢獻文章

24 引文 斯高帕斯(Scopus)

摘要

Macrophage inflammatory protein-1α (MIP-1α) has an important role in the development of inflammatory responses during infection by regulating leukocyte trafficking and function. Our study was conducted to investigate the effect of adrenaline on lipopolysaccharide (LPS)-induced MIP-1α production by human peripheral blood monocytes and human monocytic THP-1 cells. Monocytes were incubated in vitro with LPS for 4 h at 37°C in the presence and absence of adrenaline and/or specific α- and β-adrenergic receptor antagonists and agonists. The effects of adrenaline on MIP-1α synthesis were studied at the protein level by using enzyme-linked immunosorbent assays and at the messenger RNA level by using reverse transcriptasepolymerase chain reaction. Adrenaline inhibited LPSinduced MIP-1α production in a dose-dependent manner. The suppressive effect could be completely prevented by propranolol, but not by phentolamine. The specific β-adrenergic agonist isoproterenol produced the same inhibitory effect on LPS-induced MIP-1α production, whereas the α-adrenergic agonist phenylephrine had a minimal effect. In addition, suppression of MIP-1α production was associated with an increase of intracellular cyclic adenosine monophosphate (cAMP) by the cell membrane-permeable cAMP analog dibutyryl-cAMP. Furthermore, we found that adrenaline inhibited LPS-induced MIP-1α messenger RNA expression. These findings suggest that adrenaline can modulate MIP-1α production in inflammatory diseases and sepsis.

原文英語
頁(從 - 到)518-523
頁數6
期刊Anesthesia and Analgesia
96
發行號2
DOIs
出版狀態已發佈 - 二月 1 2003
對外發佈Yes

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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