Adipocytes promote malignant growth of breast tumours with monocarboxylate transporter 2 expression via β-hydroxybutyrate

Chun Kai Huang, Po Hao Chang, Wen Hung Kuo, Chi Long Chen, Yung Ming Jeng, King Jen Chang, Jin Yuh Shew, Chun Mei Hu, Wen Hwa Lee

研究成果: 雜誌貢獻文章

17 引文 (Scopus)

摘要

Adipocytes are the most abundant stromal partners in breast tissue. However, the crosstalk between breast cancer cells and adipocytes has been given less attention compared to cancer-associated fibroblasts. Here we find, through systematic screening, that primary mammary gland-derived adipocytes (MGDAs) promote growth of breast cancer cells that express monocarboxylate transporter 2 (MCT2) both in vitro and in vivo. We show that β-hydroxybutyrate is secreted by MGDAs and is required to enhance breast cancer cells malignancy in vitro. Consistently, β-hydroxybutyrate is sufficient to promote tumorigenesis of a mouse xenograft model of MCT2-expressing breast cancer cells. Mechanistically we observe that upon co-culturing with MGDAs or treatment with β-hydroxybutyrate, breast cancer cells expressing MCT2 increase the global histone H3K9 acetylation and upregulate several tumour-promoting genes. These results suggest that adipocytes promote malignancy of MCT2-expressing breast cancer via β-hydroxybutyrate potentially by inducing the epigenetic upregulation of tumour-promoting genes.
原文英語
文章編號14706
期刊Nature Communications
8
DOIs
出版狀態已發佈 - 三月 10 2017

指紋

Hydroxybutyrates
transporter
Adipocytes
breast
Tumors
tumors
cancer
Cells
Breast Neoplasms
mammary glands
Human Mammary Glands
Growth
Genes
Neoplasms
genes
Up-Regulation
Acetylation
Fibroblasts
Crosstalk
acetylation

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

引用此文

Adipocytes promote malignant growth of breast tumours with monocarboxylate transporter 2 expression via β-hydroxybutyrate. / Huang, Chun Kai; Chang, Po Hao; Kuo, Wen Hung; Chen, Chi Long; Jeng, Yung Ming; Chang, King Jen; Shew, Jin Yuh; Hu, Chun Mei; Lee, Wen Hwa.

於: Nature Communications, 卷 8, 14706, 10.03.2017.

研究成果: 雜誌貢獻文章

Huang, Chun Kai ; Chang, Po Hao ; Kuo, Wen Hung ; Chen, Chi Long ; Jeng, Yung Ming ; Chang, King Jen ; Shew, Jin Yuh ; Hu, Chun Mei ; Lee, Wen Hwa. / Adipocytes promote malignant growth of breast tumours with monocarboxylate transporter 2 expression via β-hydroxybutyrate. 於: Nature Communications. 2017 ; 卷 8.
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abstract = "Adipocytes are the most abundant stromal partners in breast tissue. However, the crosstalk between breast cancer cells and adipocytes has been given less attention compared to cancer-associated fibroblasts. Here we find, through systematic screening, that primary mammary gland-derived adipocytes (MGDAs) promote growth of breast cancer cells that express monocarboxylate transporter 2 (MCT2) both in vitro and in vivo. We show that β-hydroxybutyrate is secreted by MGDAs and is required to enhance breast cancer cells malignancy in vitro. Consistently, β-hydroxybutyrate is sufficient to promote tumorigenesis of a mouse xenograft model of MCT2-expressing breast cancer cells. Mechanistically we observe that upon co-culturing with MGDAs or treatment with β-hydroxybutyrate, breast cancer cells expressing MCT2 increase the global histone H3K9 acetylation and upregulate several tumour-promoting genes. These results suggest that adipocytes promote malignancy of MCT2-expressing breast cancer via β-hydroxybutyrate potentially by inducing the epigenetic upregulation of tumour-promoting genes.",
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AU - Jeng, Yung Ming

AU - Chang, King Jen

AU - Shew, Jin Yuh

AU - Hu, Chun Mei

AU - Lee, Wen Hwa

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