ADAM12/syndecan-4 signaling promotes β1 integrin-dependent cell spreading through protein kinase Cα and RhoA

Charles Kumar Thodeti, Reidar Albrechtsen, Morten Grauslund, Meena Asmar, Christer Larsson, Yoshikazu Takada, Arthur M. Mercurio, John R. Couchman, Ulla M. Wewer

研究成果: 雜誌貢獻文章

100 引文 斯高帕斯(Scopus)

摘要

The ADAMs (a disintegrin and metalloprotease) comprise a large family of multidomain proteins with cell-binding and metalloprotease activities. The ADAM12 cysteine-rich domain (rADAM12-cys) supports cell attachment using syndecan-4 as a primary cell surface receptor that subsequently triggers β1, integrin-dependent cell spreading, stress fiber assembly, and focal adhesion formation. This process contrasts with cell adhesion on fibronectin, which is integrin-initiated but syndecan-4-dependent. In the present study, we investigated ADAM12/syndecan-4 signaling leading to cell spreading and stress fiber formation. We demonstrate that syndecan-4, when present in significant amounts, promotes β1 integrin-dependent cell spreading and stress fiber formation in response to rADAM12-cys. A mutant form of syndecan-4 deficient in protein kinase C (PKC)α activation or a different member of the syndecan family, syndecan-2, was unable to promote cell spreading. GF109203X and Gö6976, inhibitors of PKC, completely inhibited ADAM12/syndecan-4-induced cell spreading. Expression of syndecan-4, but not syn4ΔI, resulted in the accumulation of activated, integrins at the cell periphery in Chinese hamster ovary fil 131 cells as revealed by 12G10 staining. Further, expression of myristoylated, constitutively active PKCα resulted in β1 integrin-dependent cell spreading, but additional activation of RhoA was required to induce stress fiber formation. In summary, these data provide novel insights into syndecan-4 signaling. Syndecan-4 can promote cell spreading in a β1 integrin-dependent fashion through PKCα and RhoA, and PKCα and RhoA likely function in separate pathways.

原文英語
頁(從 - 到)9576-9584
頁數9
期刊Journal of Biological Chemistry
278
發行號11
DOIs
出版狀態已發佈 - 三月 14 2003
對外發佈Yes

ASJC Scopus subject areas

  • Biochemistry

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