Acute unilateral ureteral distension inhibits glutamate-dependent spinal pelvic-urethra reflex potentiation via GABAergic neurotransmission in anesthetized rats

Kuo Jung Chen, Hsien Yu Peng, Chen Li Cheng, Cheng Hsu Chen, Jiuan Miaw Liao, Yu Cheng Ho, Jung Tong Liou, Kwong Chung Tung, Tien Huan Hsu, Tzer Bin Lin

研究成果: 雜誌貢獻文章

15 引文 (Scopus)

摘要

The effects of an acute increase in intraureteral pressure (IUP) on pelvic-urethra reflex potentiation were examined in urethane-anesthetized rats by recording the external urethral sphincter electromyogram activities evoked by the pelvic afferent stimulation. Compared with a single action potential elicited by the test stimulation (TS; characterized by an intensity that evoked a constant reflex response without facilitation, 1/30 Hz, 1.03 ± 0.12 spikes/stimulation, n = 7), the repetitive stimulation [RS; identical stimulation intensity as the TS (1 Hz)] significantly induced spinal reflex potentiation (SRP; 16.90 ± 2.00 spikes/stimulation, P <0.01, n = 7). Such SRP was significantly attenuated by intrathecal 2,3-dihydroxy-6-nitro-7- sulfamoyl-benzo (F) quinoxaline [NBQX; a glutamatergic α-amino-3-hydroxy- 5-methyl-4-isoxazoleproprionat (AMPA) receptor antagonist] and D-2-amino-5-phosphonovalerate [APV; a glutamatergic N-methyl-D-aspartate (NMDA) antagonist; the spike number per stimulation: 11.0 ± 0.70 for NBQX, 1.01 ± 0.30 for APV, and 16.90 ± 2.0 for RS, respectively, n = 7, P <0.01]. Acute stepwise elevations of IUP gradually attenuated and eventually abolished the RS-induced SRP (16.80 ± 1.30, 17.00 ± 1.30, 16.30 ± 1.30, 10.50 ± 1.80, 8.80 ± 1.90, 3.50 ± 1.60, 0.80 ± 0.20, 0.70 ± 0.20, and 0.20 ± 0.10 spikes/stimulation at intraureteral pressure of 0, 2.5, 5, 7.5, 10, 12.5, 15, 17.5, and 20 cmH 2O, respectively, n = 7). Intrathecal NMDA (a glutamatergic NMDA receptor agonist) and bicuculline (a GABA receptor antagonist) both reversed the abolition of RS-induced SRP caused by unilateral ureteral distension (14.0 ± 4.04 and 8.00 ± 1.53 spikes/stimulation, respectively, n = 7, P <0.01). All the results suggested unilateral ureteral distension might compensatorily relax the urethra via GABAergic inhibition of NMDA-dependent SRP.

原文英語
期刊American Journal of Physiology - Renal Physiology
292
發行號3
DOIs
出版狀態已發佈 - 三月 2007
對外發佈Yes

指紋

Urethra
N-Methylaspartate
Synaptic Transmission
Reflex
Glutamic Acid
Pressure
GABA Antagonists
2-Amino-5-phosphonovalerate
Quinoxalines
AMPA Receptors
Bicuculline
Urethane
Electromyography
N-Methyl-D-Aspartate Receptors
Action Potentials
2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline

ASJC Scopus subject areas

  • Physiology

引用此文

Acute unilateral ureteral distension inhibits glutamate-dependent spinal pelvic-urethra reflex potentiation via GABAergic neurotransmission in anesthetized rats. / Chen, Kuo Jung; Peng, Hsien Yu; Cheng, Chen Li; Chen, Cheng Hsu; Liao, Jiuan Miaw; Ho, Yu Cheng; Liou, Jung Tong; Tung, Kwong Chung; Hsu, Tien Huan; Lin, Tzer Bin.

於: American Journal of Physiology - Renal Physiology, 卷 292, 編號 3, 03.2007.

研究成果: 雜誌貢獻文章

Chen, Kuo Jung ; Peng, Hsien Yu ; Cheng, Chen Li ; Chen, Cheng Hsu ; Liao, Jiuan Miaw ; Ho, Yu Cheng ; Liou, Jung Tong ; Tung, Kwong Chung ; Hsu, Tien Huan ; Lin, Tzer Bin. / Acute unilateral ureteral distension inhibits glutamate-dependent spinal pelvic-urethra reflex potentiation via GABAergic neurotransmission in anesthetized rats. 於: American Journal of Physiology - Renal Physiology. 2007 ; 卷 292, 編號 3.
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title = "Acute unilateral ureteral distension inhibits glutamate-dependent spinal pelvic-urethra reflex potentiation via GABAergic neurotransmission in anesthetized rats",
abstract = "The effects of an acute increase in intraureteral pressure (IUP) on pelvic-urethra reflex potentiation were examined in urethane-anesthetized rats by recording the external urethral sphincter electromyogram activities evoked by the pelvic afferent stimulation. Compared with a single action potential elicited by the test stimulation (TS; characterized by an intensity that evoked a constant reflex response without facilitation, 1/30 Hz, 1.03 ± 0.12 spikes/stimulation, n = 7), the repetitive stimulation [RS; identical stimulation intensity as the TS (1 Hz)] significantly induced spinal reflex potentiation (SRP; 16.90 ± 2.00 spikes/stimulation, P <0.01, n = 7). Such SRP was significantly attenuated by intrathecal 2,3-dihydroxy-6-nitro-7- sulfamoyl-benzo (F) quinoxaline [NBQX; a glutamatergic α-amino-3-hydroxy- 5-methyl-4-isoxazoleproprionat (AMPA) receptor antagonist] and D-2-amino-5-phosphonovalerate [APV; a glutamatergic N-methyl-D-aspartate (NMDA) antagonist; the spike number per stimulation: 11.0 ± 0.70 for NBQX, 1.01 ± 0.30 for APV, and 16.90 ± 2.0 for RS, respectively, n = 7, P <0.01]. Acute stepwise elevations of IUP gradually attenuated and eventually abolished the RS-induced SRP (16.80 ± 1.30, 17.00 ± 1.30, 16.30 ± 1.30, 10.50 ± 1.80, 8.80 ± 1.90, 3.50 ± 1.60, 0.80 ± 0.20, 0.70 ± 0.20, and 0.20 ± 0.10 spikes/stimulation at intraureteral pressure of 0, 2.5, 5, 7.5, 10, 12.5, 15, 17.5, and 20 cmH 2O, respectively, n = 7). Intrathecal NMDA (a glutamatergic NMDA receptor agonist) and bicuculline (a GABA receptor antagonist) both reversed the abolition of RS-induced SRP caused by unilateral ureteral distension (14.0 ± 4.04 and 8.00 ± 1.53 spikes/stimulation, respectively, n = 7, P <0.01). All the results suggested unilateral ureteral distension might compensatorily relax the urethra via GABAergic inhibition of NMDA-dependent SRP.",
keywords = "Intraureteral pressure, N-methyl-D-aspartic acid, Spinal reflex potentiation, Unilateral ureteral obstruction",
author = "Chen, {Kuo Jung} and Peng, {Hsien Yu} and Cheng, {Chen Li} and Chen, {Cheng Hsu} and Liao, {Jiuan Miaw} and Ho, {Yu Cheng} and Liou, {Jung Tong} and Tung, {Kwong Chung} and Hsu, {Tien Huan} and Lin, {Tzer Bin}",
year = "2007",
month = "3",
doi = "10.1152/ajprenal.00256.2006",
language = "English",
volume = "292",
journal = "American Journal of Physiology - Renal Physiology",
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publisher = "American Physiological Society",
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T1 - Acute unilateral ureteral distension inhibits glutamate-dependent spinal pelvic-urethra reflex potentiation via GABAergic neurotransmission in anesthetized rats

AU - Chen, Kuo Jung

AU - Peng, Hsien Yu

AU - Cheng, Chen Li

AU - Chen, Cheng Hsu

AU - Liao, Jiuan Miaw

AU - Ho, Yu Cheng

AU - Liou, Jung Tong

AU - Tung, Kwong Chung

AU - Hsu, Tien Huan

AU - Lin, Tzer Bin

PY - 2007/3

Y1 - 2007/3

N2 - The effects of an acute increase in intraureteral pressure (IUP) on pelvic-urethra reflex potentiation were examined in urethane-anesthetized rats by recording the external urethral sphincter electromyogram activities evoked by the pelvic afferent stimulation. Compared with a single action potential elicited by the test stimulation (TS; characterized by an intensity that evoked a constant reflex response without facilitation, 1/30 Hz, 1.03 ± 0.12 spikes/stimulation, n = 7), the repetitive stimulation [RS; identical stimulation intensity as the TS (1 Hz)] significantly induced spinal reflex potentiation (SRP; 16.90 ± 2.00 spikes/stimulation, P <0.01, n = 7). Such SRP was significantly attenuated by intrathecal 2,3-dihydroxy-6-nitro-7- sulfamoyl-benzo (F) quinoxaline [NBQX; a glutamatergic α-amino-3-hydroxy- 5-methyl-4-isoxazoleproprionat (AMPA) receptor antagonist] and D-2-amino-5-phosphonovalerate [APV; a glutamatergic N-methyl-D-aspartate (NMDA) antagonist; the spike number per stimulation: 11.0 ± 0.70 for NBQX, 1.01 ± 0.30 for APV, and 16.90 ± 2.0 for RS, respectively, n = 7, P <0.01]. Acute stepwise elevations of IUP gradually attenuated and eventually abolished the RS-induced SRP (16.80 ± 1.30, 17.00 ± 1.30, 16.30 ± 1.30, 10.50 ± 1.80, 8.80 ± 1.90, 3.50 ± 1.60, 0.80 ± 0.20, 0.70 ± 0.20, and 0.20 ± 0.10 spikes/stimulation at intraureteral pressure of 0, 2.5, 5, 7.5, 10, 12.5, 15, 17.5, and 20 cmH 2O, respectively, n = 7). Intrathecal NMDA (a glutamatergic NMDA receptor agonist) and bicuculline (a GABA receptor antagonist) both reversed the abolition of RS-induced SRP caused by unilateral ureteral distension (14.0 ± 4.04 and 8.00 ± 1.53 spikes/stimulation, respectively, n = 7, P <0.01). All the results suggested unilateral ureteral distension might compensatorily relax the urethra via GABAergic inhibition of NMDA-dependent SRP.

AB - The effects of an acute increase in intraureteral pressure (IUP) on pelvic-urethra reflex potentiation were examined in urethane-anesthetized rats by recording the external urethral sphincter electromyogram activities evoked by the pelvic afferent stimulation. Compared with a single action potential elicited by the test stimulation (TS; characterized by an intensity that evoked a constant reflex response without facilitation, 1/30 Hz, 1.03 ± 0.12 spikes/stimulation, n = 7), the repetitive stimulation [RS; identical stimulation intensity as the TS (1 Hz)] significantly induced spinal reflex potentiation (SRP; 16.90 ± 2.00 spikes/stimulation, P <0.01, n = 7). Such SRP was significantly attenuated by intrathecal 2,3-dihydroxy-6-nitro-7- sulfamoyl-benzo (F) quinoxaline [NBQX; a glutamatergic α-amino-3-hydroxy- 5-methyl-4-isoxazoleproprionat (AMPA) receptor antagonist] and D-2-amino-5-phosphonovalerate [APV; a glutamatergic N-methyl-D-aspartate (NMDA) antagonist; the spike number per stimulation: 11.0 ± 0.70 for NBQX, 1.01 ± 0.30 for APV, and 16.90 ± 2.0 for RS, respectively, n = 7, P <0.01]. Acute stepwise elevations of IUP gradually attenuated and eventually abolished the RS-induced SRP (16.80 ± 1.30, 17.00 ± 1.30, 16.30 ± 1.30, 10.50 ± 1.80, 8.80 ± 1.90, 3.50 ± 1.60, 0.80 ± 0.20, 0.70 ± 0.20, and 0.20 ± 0.10 spikes/stimulation at intraureteral pressure of 0, 2.5, 5, 7.5, 10, 12.5, 15, 17.5, and 20 cmH 2O, respectively, n = 7). Intrathecal NMDA (a glutamatergic NMDA receptor agonist) and bicuculline (a GABA receptor antagonist) both reversed the abolition of RS-induced SRP caused by unilateral ureteral distension (14.0 ± 4.04 and 8.00 ± 1.53 spikes/stimulation, respectively, n = 7, P <0.01). All the results suggested unilateral ureteral distension might compensatorily relax the urethra via GABAergic inhibition of NMDA-dependent SRP.

KW - Intraureteral pressure

KW - N-methyl-D-aspartic acid

KW - Spinal reflex potentiation

KW - Unilateral ureteral obstruction

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