Acute neurosteroids inhibit the spinal reflex potentiation via GABAergic neurotransmission

Junn Liang Chang, Hsien Yu Peng, Hsi Chin Wu, Hsiao Ting Lu, Shwu Fen Pan, Mei Jung Chen, Tzer Bin Lin

研究成果: 雜誌貢獻文章

7 引文 斯高帕斯(Scopus)

摘要

Recently, we demonstrated a chronic neurosteroid-dependent inhibition of activity-dependent spinal reflex potentiation (SRP), but it remains unclear whether neurosteroids acutely modulate SRP induction. This study shows progesterone as well as two of its 3α,5α-derivatives, allopregnalonone and 3α,5α-tetrahydrodeoxycorticosterone (THDOC), to be capable of producing acute GABAA receptor (GABA AR)-dependent inhibition of SRP. When compared with test simulation (1 stimulation/30 s) of pelvic afferent nerves that evoked a baseline reflex activity in an external urethra sphincter electromyogram, repetitive stimulation (RS; 1 stimulation/1 s) induced SRP characterized by an increase in the evoked activity. Intrathecal progesterone (3-30 μM, 10 μl) at 10 min before stimulation onset dose dependently prevented RS induction. Intrathecal allopregnalonone (10 μM, 10 μl it) and THDOC (10 μM, 10 μl it) also prevented the SRP caused by RS. Pretreatment with the GABAAR antagonist bicuculline (10 μM, 10 μl it) at 1 min before progesterone/neurosteroid injection attenuated the inhibition of SRP caused by progesterone, allopregnanolone, and THDOC. Results suggest that progesterone and its neurosteroid metabolites may be crucial to the development of pelvic visceral neuropathic/postinflammatory pain and imply clinical use of neurosteroids, such as allopregnanolone and THDOC, for visceral pain treatment.

原文英語
期刊American Journal of Physiology - Renal Physiology
299
發行號1
DOIs
出版狀態已發佈 - 七月 2010
對外發佈Yes

ASJC Scopus subject areas

  • Physiology
  • Urology

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