Acute anal stretch inhibits NMDA-dependent pelvic-urethra reflex potentiation via spinal GABAergic inhibition in anesthetized rats

Sung Lang Chen, Yu Hui Huang, Yu Lin Kao, Gin Den Chen, Chen Li Cheng, Hsien Yu Peng, Jiuan Miaw Liao, Pei Chen Huang, Shih Jei Tsai, Tzer Bin Lin

研究成果: 雜誌貢獻文章

6 引文 斯高帕斯(Scopus)

摘要

The impact of acute anal stretch on the pelvic-urethra reflex potentiation was examined in urethane-anesthetized rats by recording the external urethra sphincter electromyogram activity evoked by the pelvic afferent stimulation. Test stimulation (1 stimulation/30 s) evoked a baseline reflex activity with a single action potential that was abolished by gallamine (5 mg/kg iv). On the other hand, the repetitive stimulation (1 stimulation/1 s) induced spinal reflex potentiation (SRP) that was attenuated by intrathecal 6-cyano-7- nitroquinoxaline-2,4-dione (a glutamatergic α-amino-3-hydroxy-5-methyl-4- isoxazoleproprionat receptor antagonist, 100 μM, 10 μl) and D-2-amino-5-phosphonovalerate [a glutamatergic N-methyl-D-aspartate (NMDA) antagonist, 100 μM, 10 μl]. Acute anal stretch using a mosquito clamp with a distance of 4 mm exhibited no effect, whereas distances of 8 mm attenuated and 12 mm abolished the repetitive stimulation-induced SRP. Intrathecal NMDA (100 μM, 10 μl) reversed the abolition on SRP caused by anal stretch. On the other hand, pretreated bicuculline [γ-aminobutyric acid (GABA) A receptor antagonist, 100 μM, 10 μl] but not hydroxysaclofen (GABAB receptor antagonist) counteracted the abolition on the repetitive stimulation-induced SRP caused by the anal stretch. All of the results suggested that anal stretch may be used as an adjunct to assist voiding dysfunction in patients with overactive urethra sphincter and that GABAergic neurotransmission is important in the neural mechanisms underlying external urethra sphincter activity inhibited by anal stretch.

原文英語
期刊American Journal of Physiology - Renal Physiology
295
發行號4
DOIs
出版狀態已發佈 - 十月 2008
對外發佈Yes

ASJC Scopus subject areas

  • Physiology
  • Urology

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