摘要
Pectinesterase (PE), from commercial orange peels or ammonium sulfate fractionation (50-80% saturation) of pea pods, was detected on polyacrylamide gels after native acidic polyacrylamide gel electrophoresis (PAGE) or sodium dodecyl sulfate (SDS)-PAGE by using the synthetic substrate β-naphthyl acetate (β-NA). The release of β-naphthol (at 322 nm) from β-NA was proportional to PE activity. The PE activity bands on polyacrylamide gels after native acidic PAGE or SDS-PAGE were stained with a combination of tetrazotized o-dianisidine and β-NA. This fast and sensitive method can be used for enzyme purification and characterization.
原文 | 英語 |
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頁(從 - 到) | 692-694 |
頁數 | 3 |
期刊 | Electrophoresis |
卷 | 19 |
發行號 | 5 |
DOIs | |
出版狀態 | 已發佈 - 5月 1998 |
對外發佈 | 是 |
ASJC Scopus subject areas
- 臨床生物化學