Activity staining of pectinesterase on-polyacrylamide gels after acidic or sodium dodecyl sulfate electrophoresis

Wen Chi Hou, Yaw Huei Lin

研究成果: 雜誌貢獻文章

17 引文 斯高帕斯(Scopus)

摘要

Pectinesterase (PE), from commercial orange peels or ammonium sulfate fractionation (50-80% saturation) of pea pods, was detected on polyacrylamide gels after native acidic polyacrylamide gel electrophoresis (PAGE) or sodium dodecyl sulfate (SDS)-PAGE by using the synthetic substrate β-naphthyl acetate (β-NA). The release of β-naphthol (at 322 nm) from β-NA was proportional to PE activity. The PE activity bands on polyacrylamide gels after native acidic PAGE or SDS-PAGE were stained with a combination of tetrazotized o-dianisidine and β-NA. This fast and sensitive method can be used for enzyme purification and characterization.

原文英語
頁(從 - 到)692-694
頁數3
期刊Electrophoresis
19
發行號5
DOIs
出版狀態已發佈 - 五月 1998
對外發佈Yes

ASJC Scopus subject areas

  • Clinical Biochemistry

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