Activity staining of isocitrate lyase after electrophoresis on either native or sodium dodecyl sulfate polyacrylamide gels

Wen Chi Hou, Hsien Jung Chen, Yaw Huei Lin, Yen Chou Chen, Ling-Ling Yang, Mei Hsien Lee

研究成果: 雜誌貢獻文章

6 引文 斯高帕斯(Scopus)

摘要

Isocitrate was cleaved into succinate and glyoxylate by isocitrate lyase (ICL) in the glyoxylate cycle. We used lactate dehydrogenase as an ancillary enzyme to further metabolize the glyoxylate to glycolate in the presence of NADH. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 2,6-dichlorophenol-indolphenol (DCPIP) were used in the coupling reactions for detecting ICL activity after electrophoresis on either native or sodium dodecyl sulfate (SDS) polyacrylamide gels. This fast and sensitive method can be used in the process of ICL enzyme purification and characterization.

原文英語
頁(從 - 到)2653-2655
頁數3
期刊Electrophoresis
22
發行號13
DOIs
出版狀態已發佈 - 2001

ASJC Scopus subject areas

  • Clinical Biochemistry

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