Activation of a novel ubiquitin-independent proteasome pathway when RNA polymerase II encounters a protein roadblock

Yi Ban, Chia Wen Ho, Ren Kuo Lin, Yi Lisa Lyu, Leroy F. Liu

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18 引文 斯高帕斯(Scopus)

摘要

Topoisomerase IIβ (Top2β)-DNA cleavage complexes are known to arrest elongating RNA polymerase II (RNAPII), triggering a proteasomal degradation of the RNAPII large subunit (RNAPII LS) and Top2β itself as a prelude to DNA repair. Here, we demonstrate that the degradation of Top2β occurs through a novel ubiquitin-independent mechanism that requires only 19S AAA ATPases and 20S proteasome. Our results suggest that 19S AAA ATPases play a dual role in sensing the Top2β cleavage complex and coordinating its degradation by 20S proteasome when RNAPII is persistently stalled by the Top2β protein roadblock. Clarification of this transcription-associated proteasome pathway could shed light on a general role of 19S AAA ATPases in processing tight protein-DNA complexes during transcription elongation.

原文英語
頁(從 - 到)4008-4016
頁數9
期刊Molecular and Cellular Biology
33
發行號20
DOIs
出版狀態已發佈 - 2013

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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