Activation and up-regulation of nitric oxide synthase in human umbilical vein endothelial cells by polycyclic aromatic hydrocarbons

Ching H. Li, Chen Chen Lee, Yu W. Cheng, Hwe A. Juang, Jaw J. Kang

研究成果: 雜誌貢獻文章

11 引文 斯高帕斯(Scopus)

摘要

PAHs, including naphthalene, fluoranthene and fluorene rapidly induced extracellular Ca2+ influx and hence elevation of intracellular Ca2+ concentration and NO production. The effect can be inhibited by Ca2+ channel blocker but not by P450 inhibitor. In addition to the rapid effect, we have also found that the eNOS mRNA and protein expression were augmented in HUVECs treated with PAHs at concentration as low as 0.1 μM for 24 h. These effects were abolished when the HUVECs were pretreated with the BAPTA, NiCl2 and SKF96365, as well as SKF525A. Our results revealed that, for the first time, PAHs induce the activation of eNOS and enhance eNOS protein expression in HUVECs both in a Ca2+-dependent manner.
原文英語
頁(從 - 到)367-374
頁數8
期刊Toxicology Letters
151
發行號2
DOIs
出版狀態已發佈 - 七月 15 2004

ASJC Scopus subject areas

  • Toxicology

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