Activated type I phosphatidylinositol kinase is associated with the epidermal growth factor (EGF) receptor following EGF stimulation

Jeffrey D. Bjorge, Tung On Chan, Michael Antczak, Hsing Jien Kung, Donald J. Fujita

研究成果: 雜誌貢獻文章同行評審

132 引文 斯高帕斯(Scopus)

摘要

We have shown that a type I phosphatidylinositol (PI) kinase activity is associated with the epidermal growth factor (EGF) receptor in a mouse fibroblast cell line expressing human EGF receptors (NRHER5) and that this activity increases dramatically upon treatment of cells with physiologically relevant concentrations of EGF. EGF stimulated a time-dependent increase in EGF receptor-associated PI kinase activity measured in EGF receptor immunoprecipitates. Activation was detected 15 min after the addition of EGF, and it peaked between 1 and 2 hr. Activation of PI kinase was detected with EGF concentrations as low as 10 pM and maximal stimulation occurred at ≈1 nM. Analysis of deacylated PI phosphate products, and inhibition of the PI kinase activity by nonionic detergent, indicated that the PI kinase described here was type I or PI 3′ kinase. These results demonstrate the regulation of a type I PI kinase by EGF and suggest a potential role in the EGF receptor signal transduction pathway. (.
原文英語
頁(從 - 到)3816-3820
頁數5
期刊Proceedings of the National Academy of Sciences of the United States of America
87
發行號10
DOIs
出版狀態已發佈 - 1月 1 1990
對外發佈

ASJC Scopus subject areas

  • 多學科

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