Activated conformations of very late activation integrins detected by a group of antibodies (HUTS) specific for a novel regulatory region (355-425) of the common β1 chain

Alfonso Luque, Manuel Gómez, Wilma Puzon, Yoshikazu Takada, Francisco Sánchez-Madrid, Carlos Cabañas

研究成果: 雜誌貢獻文章同行評審

249 引文 斯高帕斯(Scopus)

摘要

The very late activation antigens (VLA) or β1 integrins mediate cell attachment to different extracellular matrix proteins and intercellular adhesions. The ligand binding activity of these adhesion receptors is not constitutive and can be regulated by temperature, presence of extracellular divalent cations, stimulatory monoclonal antibodies (mAbs), and cellular activation. We have generated three novel mAbs, HUTS-4, HUTS-7, and HUTS-21, recognizing specific epitopes on the common β1 subunit (CD29) of VLA integrins whose expression correlates with the ligand binding activity of these heterodimeric glycoproteins. This correlation has been demonstrated for several integrin heterodimers in different cell systems using a variety of extracellular and intracellular stimuli for integrin activation. Thus, the presence of micromolar concentrations of extracellular Mn2+, preincubation with the activating anti-β1 mAb TS2/16, and cell treatment with phorbol esters or calcium ionophores, induced the expression of the HUTS β1 epitopes on T lymphoblasts. Using a panel of human-mouse β1 chimeric molecules, we have mapped these epitopes to the 355-425 sequence of the β1 polypeptide. This segment represents therefore a novel regulatory region of β1 that is exposed upon integrin activation. Interestingly, binding of HUTS mAbs to partially activated VLA integrins results in maximal activation of these adhesion receptors and enhancement of cell adhesion to β1 integrin ligands collagen, laminin, and fibronectin.

原文英語
頁(從 - 到)11067-11075
頁數9
期刊Journal of Biological Chemistry
271
發行號19
DOIs
出版狀態已發佈 - 1996

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

指紋 深入研究「Activated conformations of very late activation integrins detected by a group of antibodies (HUTS) specific for a novel regulatory region (355-425) of the common β1 chain」主題。共同形成了獨特的指紋。

引用此