Abortifacient Effects of a Unique Class of Vasoactive Lipids from Pinus ponderosa Needles

S. P. Ford, J. P N Rosazza, M. S. Al-Mahmoud, S. Lin, D. B. Farley, R. E. Short

研究成果: 雜誌貢獻文章

6 引文 斯高帕斯(Scopus)

摘要

Pinus ponderosa needle (PN) ingestion by late pregnant cows results in decreased uterine blood flow, premature parturition, and retained placentae. Further, plasma from PN-fed cows increases caruncular arterial tone (i.e., induces prolonged contraction) in an isolated perfused bovine placentome. A novel class of vasoactive lipids was isolated and identified using a bovine placentome assay-guided fractionation of CH2Cl2 extracts of PN. Placentome perfusion tests indicated that 1-12-dodecanedioyl-dimyristate (14-12-14) was the most potent of the PN lipids for increasing caruncular arterial tone. Late pregnant guinea pigs (GP) were used to evaluate the abortifacient activity of these vasoactive lipids. In Study 1, on d 50 of gestation, part of the control diet was replaced with chopped PN (Diet A) or chopped PN subjected to sequential extraction with diethyl ether (Et20; Diet B); Et20 and CH2Cl2 (Diet C); and Et2O, CH2Cl2, and methanol (Diet D). The GP on Diets A and B exhibited shorter (P <.01) gestation lengths and reduced (P <.01) pig birth weights than GP on the control diet or Diets C and D. Further, only GP on Diets A and B exhibited retained placentae. In Study 2, on d 50 of gestation, part of the control diet was replaced with chopped PN that had been subjected to exhaustive CH2Cl2 extraction and then infiltrated with either CH2Cl2 alone (Diet E), CH2Cl2 containing 14-12-14 (Diet F), or CH2Cl2 containing isocupressic acid (Diet G); then solvents were evaporated. The GP consuming Diet F had shorter (P <.05) gestation lengths and reduced (P <.05) pig birth weights than did GP consuming Diets E or G. The GP consuming Diet F also exhibited a high incidence of retained placentae. These data provide evidence that a unique class of vasoactive lipids in PN exhibit abortifacient activity in guinea pigs.
原文英語
頁(從 - 到)2187-2193
頁數7
期刊Journal of Animal Science
77
發行號8
出版狀態已發佈 - 八月 1999
對外發佈Yes

ASJC Scopus subject areas

  • Animal Science and Zoology

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    Ford, S. P., Rosazza, J. P. N., Al-Mahmoud, M. S., Lin, S., Farley, D. B., & Short, R. E. (1999). Abortifacient Effects of a Unique Class of Vasoactive Lipids from Pinus ponderosa Needles. Journal of Animal Science, 77(8), 2187-2193.