Abnormalities in structure and expression of the human retinoblastoma gene in SCLC

J. W. Harbour, Shinn Liang Lai, Jacqueline Whang-Peng, Adi F. Gazdar, John D. Minna, Frederic J. Kaye

研究成果: 雜誌貢獻文章

705 引文 斯高帕斯(Scopus)

摘要

Small cell lung cancer (SCLC) has been associated with loss of heterozygosity at several distinct genetic loci including chromosomes 3p, 13q, and 17p. To determine whether the retinoblastoma gene (Rb) localized at 13q14, might be the target of recessive mutations in lung cancer, eight primary SCLC tumors and 50 cell lines representing all major histologic types of lung cancer were examined with the Rb complementary DNA probe. Structural abnormalities within the Rb gene were observed in 1/8 (13%) primary SCLC tumors, 4/22 (18%) SCLC lines, and 1/4 (25%) pulmonary carcinoid lines (comparable to the 20 to 40% observed in retinoblastoma), but were not detected in other major types of lung cancer. Rb messenger RNA expression was absent in 60% of the SCLC lines and 75% of pulmonary carcinoid lines, including all samples with DNA abnormalities. In contrast, Rb transcripts were found in 90% of non-SCLC lung cancer lines and in normal human lung. The finding of abnormalities of the Rb gene in SCLC and pulmonary carcinoids (both neuroendocrine tumors) suggests that this gene may be involved in the pathogenesis of a common adult malignancy.
原文英語
頁(從 - 到)353-357
頁數5
期刊Science
241
發行號4863
出版狀態已發佈 - 一月 1 1988
對外發佈Yes

ASJC Scopus subject areas

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    Harbour, J. W., Lai, S. L., Whang-Peng, J., Gazdar, A. F., Minna, J. D., & Kaye, F. J. (1988). Abnormalities in structure and expression of the human retinoblastoma gene in SCLC. Science, 241(4863), 353-357.