Abnormal sensory protein expression and urothelial dysfunction in ketamine-related cystitis in humans

Yao Chou Tsai, Lori Birder, Hann Chorng Kuo

研究成果: 雜誌貢獻文章

4 引文 斯高帕斯(Scopus)

摘要

Purpose: The aim of this study was to analyze patterns of sensory protein expression and urothelial dysfunction in ketaminerelated cystitis (KC) in humans. Methods: Biopsies of bladder mucosa were performed in 29 KC patients during cystoscopy. Then specimens were analyzed for tryptase, zonula occludens-1 (ZO-1), E-cadherin, and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) with immunofluorescence staining and quantification. In addition, 10 healthy control bladder specimens were analyzed and compared with the KC specimens. Another 16 whole bladder specimens obtained from partial cystectomy were also analyzed for the muscarinic receptors M2 and M3, endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS), β-3 adrenergic receptors (β3-ARs), and the P2X3 receptor by western blotting. In addition, 3 normal control bladder specimens were analyzed and compared with the KC specimens. Results: The KC bladder mucosa revealed significantly less expression of ZO-1 and E-cadherin, and greater expression of TUNEL and tryptase activity than the control samples. The expression of M3 and β3-AR in the KC specimens was significantly greater than in the controls. The expression of iNOS, eNOS, M2, and P2X3 was not significantly different between the KC and control specimens. Conclusions: The bladder tissue of KC patients revealed significant urothelial dysfunction, which was associated with mastcell mediated inflammation, increased urothelial cell apoptosis, and increased expression of the M3 and β3-AR.

原文英語
頁(從 - 到)197-202
頁數6
期刊International Neurourology Journal
20
發行號3
DOIs
出版狀態已發佈 - 2016
對外發佈Yes

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Urology

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