Aberrant cytokines/chemokines production correlate with proteinuria in patients with overt diabetic nephropathy

Chia Chao Wu, Jin Shuen Chen, Kuo Cheng Lu, Chun Chi Chen, Shih Hua Lin, Pauling Chu, Huey Kang Sytwu, Yuh Feng Lin

研究成果: 雜誌貢獻文章

47 引文 (Scopus)

摘要

Background: Diabetic nephropathy (DN) occurs in 20% to 30% of all patients with type 2 diabetes mellitus (DM) and is the most common cause of end-stage renal disease. However, the definite pathogenesis, especially the role of immune response, is still unclear. Methods: We studied the production and expression of Th1 (IFN-γ, IL-2R), Th2 (IL-4, IL-10), proinflammatory cytokines (IL-1β, and TNF-α), and chemokines (MCP-1, and RANTES) in patients with DN. The correlation among cytokines, chemokines, and clinical parameters were examined. Results: A patient with DN presented with longer disease duration, heavy proteinuria, and impaired renal function. Our results demonstrated increased proinflammatory cytokines, Th1 cytokines and chemokines, but not Th2 cytokines, in the plasma and urine of patients with DN as compared to patients with DM without overt nephropathy. Enhanced cytokine/chemokine activation in DN was also demonstrated by positive immunohistochemical staining of kidney tissue. We found a positive correlation between daily protein loss and plasma IFN-γ and IL-2R, and urinary MCP-1, as well as a negative correlation between creatinine clearance and plasma TNF-α and urinary MCP-1. Conclusions: There were aberrant cytokines/chemokines production correlated with the degree of proteinuria in patient with overt DN and gross proteinuria. Inflammation may be important in the pathogenesis of DN.

原文英語
頁(從 - 到)700-704
頁數5
期刊Clinica Chimica Acta
411
發行號9-10
DOIs
出版狀態已發佈 - 2010

指紋

Diabetic Nephropathies
Proteinuria
Chemokines
Cytokines
Medical problems
Plasmas
Kidney
Chemokine CCL5
Interleukin-1
Interleukin-4
Interleukin-10
Type 2 Diabetes Mellitus
Chronic Kidney Failure
Blood Proteins
Creatinine
Diabetes Mellitus
Chemical activation
Urine
Tissue
Staining and Labeling

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

引用此文

Aberrant cytokines/chemokines production correlate with proteinuria in patients with overt diabetic nephropathy. / Wu, Chia Chao; Chen, Jin Shuen; Lu, Kuo Cheng; Chen, Chun Chi; Lin, Shih Hua; Chu, Pauling; Sytwu, Huey Kang; Lin, Yuh Feng.

於: Clinica Chimica Acta, 卷 411, 編號 9-10, 2010, p. 700-704.

研究成果: 雜誌貢獻文章

Wu, Chia Chao ; Chen, Jin Shuen ; Lu, Kuo Cheng ; Chen, Chun Chi ; Lin, Shih Hua ; Chu, Pauling ; Sytwu, Huey Kang ; Lin, Yuh Feng. / Aberrant cytokines/chemokines production correlate with proteinuria in patients with overt diabetic nephropathy. 於: Clinica Chimica Acta. 2010 ; 卷 411, 編號 9-10. 頁 700-704.
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abstract = "Background: Diabetic nephropathy (DN) occurs in 20{\%} to 30{\%} of all patients with type 2 diabetes mellitus (DM) and is the most common cause of end-stage renal disease. However, the definite pathogenesis, especially the role of immune response, is still unclear. Methods: We studied the production and expression of Th1 (IFN-γ, IL-2R), Th2 (IL-4, IL-10), proinflammatory cytokines (IL-1β, and TNF-α), and chemokines (MCP-1, and RANTES) in patients with DN. The correlation among cytokines, chemokines, and clinical parameters were examined. Results: A patient with DN presented with longer disease duration, heavy proteinuria, and impaired renal function. Our results demonstrated increased proinflammatory cytokines, Th1 cytokines and chemokines, but not Th2 cytokines, in the plasma and urine of patients with DN as compared to patients with DM without overt nephropathy. Enhanced cytokine/chemokine activation in DN was also demonstrated by positive immunohistochemical staining of kidney tissue. We found a positive correlation between daily protein loss and plasma IFN-γ and IL-2R, and urinary MCP-1, as well as a negative correlation between creatinine clearance and plasma TNF-α and urinary MCP-1. Conclusions: There were aberrant cytokines/chemokines production correlated with the degree of proteinuria in patient with overt DN and gross proteinuria. Inflammation may be important in the pathogenesis of DN.",
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AU - Lin, Shih Hua

AU - Chu, Pauling

AU - Sytwu, Huey Kang

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N2 - Background: Diabetic nephropathy (DN) occurs in 20% to 30% of all patients with type 2 diabetes mellitus (DM) and is the most common cause of end-stage renal disease. However, the definite pathogenesis, especially the role of immune response, is still unclear. Methods: We studied the production and expression of Th1 (IFN-γ, IL-2R), Th2 (IL-4, IL-10), proinflammatory cytokines (IL-1β, and TNF-α), and chemokines (MCP-1, and RANTES) in patients with DN. The correlation among cytokines, chemokines, and clinical parameters were examined. Results: A patient with DN presented with longer disease duration, heavy proteinuria, and impaired renal function. Our results demonstrated increased proinflammatory cytokines, Th1 cytokines and chemokines, but not Th2 cytokines, in the plasma and urine of patients with DN as compared to patients with DM without overt nephropathy. Enhanced cytokine/chemokine activation in DN was also demonstrated by positive immunohistochemical staining of kidney tissue. We found a positive correlation between daily protein loss and plasma IFN-γ and IL-2R, and urinary MCP-1, as well as a negative correlation between creatinine clearance and plasma TNF-α and urinary MCP-1. Conclusions: There were aberrant cytokines/chemokines production correlated with the degree of proteinuria in patient with overt DN and gross proteinuria. Inflammation may be important in the pathogenesis of DN.

AB - Background: Diabetic nephropathy (DN) occurs in 20% to 30% of all patients with type 2 diabetes mellitus (DM) and is the most common cause of end-stage renal disease. However, the definite pathogenesis, especially the role of immune response, is still unclear. Methods: We studied the production and expression of Th1 (IFN-γ, IL-2R), Th2 (IL-4, IL-10), proinflammatory cytokines (IL-1β, and TNF-α), and chemokines (MCP-1, and RANTES) in patients with DN. The correlation among cytokines, chemokines, and clinical parameters were examined. Results: A patient with DN presented with longer disease duration, heavy proteinuria, and impaired renal function. Our results demonstrated increased proinflammatory cytokines, Th1 cytokines and chemokines, but not Th2 cytokines, in the plasma and urine of patients with DN as compared to patients with DM without overt nephropathy. Enhanced cytokine/chemokine activation in DN was also demonstrated by positive immunohistochemical staining of kidney tissue. We found a positive correlation between daily protein loss and plasma IFN-γ and IL-2R, and urinary MCP-1, as well as a negative correlation between creatinine clearance and plasma TNF-α and urinary MCP-1. Conclusions: There were aberrant cytokines/chemokines production correlated with the degree of proteinuria in patient with overt DN and gross proteinuria. Inflammation may be important in the pathogenesis of DN.

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