A technique for relating long-range base pairing on single-stranded DNA and eukaryotic RNA processing

Che Kun James Shen, John E. Hearst

研究成果: 雜誌貢獻文章同行評審

10 引文 斯高帕斯(Scopus)

摘要

Our previous results have shown that after photochemical crosslinking with psoralens in solutions of different concentrations of NaCl, short (100-300 base pairs) hairpins can be stabilized on denatured Simian virus 40 (SV40) DNA for electron microscope visualization. Six major hairpins were mapped. This study has been extended by crosslinking single stranded SV40 linear molecules, EcoRI-SV40 and Bgl I-SV40, in the presence of magnesium ions. After the reaction, in addition to the hairpins detected before, seven DNA hairpins, and hairpin loops with sizes from 300 to nearly 2000 nucleotides are observed. These loops are located at specific regions on the SV40 genome; at the EcoRI map positions (0.16 ± 0.02) (0.26 ± 0.03), (0.33 ± 0.03) (0.39 ± 0.03), (0.51 ± 0.03) (0.57 ± 0.03) (0.72 ± 0.03) (0.78 ± 0.04), (0.74 ± 0.03) (0.85 ± 0.03), (0.77 ± 0.05) (0.94 ± 0.03), and at (0.74 ± 0.04) (0.09 ± 0.07). At least three of these loops are found in regions thought to be involved in splicing of the early and late SV40 transcripts: (0.51 ± 0.03) (0.57 ± 0.03), (0.72 ± 0.03) (0.78 ± 0.04), (0.77 ± 0.05) (0.94 ± 0.03). These loop structures are most likely to arise from base pairing between distant regions of the single-stranded DNA, RNA transcribed from the double-stranded DNA template is expected to behave in a similar way, possibly providing recognition sites for processing (splicing) enzymes.

原文英語
頁(從 - 到)108-116
頁數9
期刊Analytical Biochemistry
95
發行號1
DOIs
出版狀態已發佈 - 五月 1979
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 生物化學
  • 分子生物學
  • 細胞生物學

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