A study of immunomodulatory genes responses to macrophages of Schistosoma japonicum infection during different stages by microarray analysis

Po Ching Cheng, Ching Nan Lin, Shih Yi Peng, Li Li Li, Tsai Yueh Luo, Chia Kwung Fan, Kin Mu Lee

研究成果: 雜誌貢獻文章

8 引文 (Scopus)

摘要

Macrophages initiate, modulate, and also serve as final effector cells in immune responses during the course of schistosomal infections. In this study, we investigated the gene expression profile and functional changes of macrophages in immune responses against the Schistosoma japonicum by microarray analysis. Hierarchical clustering analysis demonstrated that a significant switch in gene transformation associated with a type-1 response and linked with a type-2 cytokine phenotype occurs between 4.5 and 8 weeks post-infection. Moreover, the gene profiles at 3 later time-points following egg challenge were similar in complexity and magnitude. The data also showed that there were mostly inhibition of gene expression related TLR, IFN, MHC and TNFrsf at the switch between 4.5 and 8 weeks post-infection, It is suggested that these immunomodulatory genes may be down-regulated in defense against S. japonicum eggs and granuloma pathology. The induction of alternatively activated macrophage (AAMφ) was important for dampening the inflammation in hepatic granulomas and contributing to a decrease in cytotoxicity. The gene expressions involved in repair/remodeling during liver fibrosis were also observed after egg production. Understanding the immune mechanisms associated with parasitic resistance, pathology of parasite infection, and parasite growth will provide useful insight on host-schistosome interactions and for the control of schistosomiasis.
原文英語
頁(從 - 到)251-260
頁數10
期刊Acta Tropica
127
發行號3
DOIs
出版狀態已發佈 - 九月 2013

指紋

Schistosoma japonicum
Microarray Analysis
macrophages
Macrophages
Granuloma
Ovum
granuloma
Infection
Switch Genes
infection
gene expression
Pathology
Genes
Gene Expression
Parasitic Diseases
genes
Schistosomiasis
immune response
Transcriptome
parasites

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

引用此文

A study of immunomodulatory genes responses to macrophages of Schistosoma japonicum infection during different stages by microarray analysis. / Cheng, Po Ching; Lin, Ching Nan; Peng, Shih Yi; Li, Li Li; Luo, Tsai Yueh; Fan, Chia Kwung; Lee, Kin Mu.

於: Acta Tropica, 卷 127, 編號 3, 09.2013, p. 251-260.

研究成果: 雜誌貢獻文章

Cheng, Po Ching ; Lin, Ching Nan ; Peng, Shih Yi ; Li, Li Li ; Luo, Tsai Yueh ; Fan, Chia Kwung ; Lee, Kin Mu. / A study of immunomodulatory genes responses to macrophages of Schistosoma japonicum infection during different stages by microarray analysis. 於: Acta Tropica. 2013 ; 卷 127, 編號 3. 頁 251-260.
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