A simple, rapid, high-resolution chromosome technic for lymphocytes

Y. S. Kao, J. Whang-Peng, E. Lee

研究成果: 雜誌貢獻 › 文章

5 引文 (Scopus)

摘要

Phytohemagglutinin (PHA) stimulated lymphocytes were exposed to a hypotonic solution consisting of equal parts of 0.075 M 2-Mercaptoethanol and 0.075 M KCl, followed by addition of colcemid. The cells were fixed and washed in the usual manner. The slides then were subjected to trypsin-Giemsa banding. This method yields a high percentage of mitotic figures in prophase and prometaphase, ideal for high-resolution chromosome analysis.
原文英語
頁（從 - 到）481-483
頁數3
期刊American Journal of Clinical Pathology
卷79
發行號4
DOIs
出版狀態已發佈 - 一月 1 1983
對外發佈Yes

指紋

Hypotonic Solutions
Prometaphase
Demecolcine
Prophase
Mercaptoethanol
Phytohemagglutinins
Trypsin
Chromosomes
Lymphocytes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

引用此文

A simple, rapid, high-resolution chromosome technic for lymphocytes. / Kao, Y. S.; Whang-Peng, J.; Lee, E.

於: American Journal of Clinical Pathology, 卷 79, 編號 4, 01.01.1983, p. 481-483.

研究成果: 雜誌貢獻 › 文章

Kao, YS, Whang-Peng, J & Lee, E 1983, 'A simple, rapid, high-resolution chromosome technic for lymphocytes', American Journal of Clinical Pathology, 卷 79, 編號 4, 頁 481-483. https://doi.org/10.1093/ajcp/79.4.481
Kao YS, Whang-Peng J, Lee E. A simple, rapid, high-resolution chromosome technic for lymphocytes. American Journal of Clinical Pathology. 1983 1月 1;79(4):481-483. https://doi.org/10.1093/ajcp/79.4.481
Kao, Y. S. ; Whang-Peng, J. ; Lee, E. / A simple, rapid, high-resolution chromosome technic for lymphocytes. 於: American Journal of Clinical Pathology. 1983 ; 卷 79, 編號 4. 頁 481-483.
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