TY - JOUR
T1 - A simple, rapid, high-resolution chromosome technic for lymphocytes
AU - Kao, Y. S.
AU - Whang-Peng, J.
AU - Lee, E.
PY - 1983/1/1
Y1 - 1983/1/1
N2 - Phytohemagglutinin (PHA) stimulated lymphocytes were exposed to a hypotonic solution consisting of equal parts of 0.075 M 2-Mercaptoethanol and 0.075 M KCl, followed by addition of colcemid. The cells were fixed and washed in the usual manner. The slides then were subjected to trypsin-Giemsa banding. This method yields a high percentage of mitotic figures in prophase and prometaphase, ideal for high-resolution chromosome analysis.
AB - Phytohemagglutinin (PHA) stimulated lymphocytes were exposed to a hypotonic solution consisting of equal parts of 0.075 M 2-Mercaptoethanol and 0.075 M KCl, followed by addition of colcemid. The cells were fixed and washed in the usual manner. The slides then were subjected to trypsin-Giemsa banding. This method yields a high percentage of mitotic figures in prophase and prometaphase, ideal for high-resolution chromosome analysis.
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U2 - 10.1093/ajcp/79.4.481
DO - 10.1093/ajcp/79.4.481
M3 - Article
C2 - 6837512
AN - SCOPUS:0020630316
VL - 79
SP - 481
EP - 483
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
SN - 0002-9173
IS - 4
ER -