A simple, rapid, high-resolution chromosome technic for lymphocytes

Y. S. Kao; J. Whang-Peng; E. Lee

doi:10.1093/ajcp/79.4.481

A simple, rapid, high-resolution chromosome technic for lymphocytes

Y. S. Kao, J. Whang-Peng, E. Lee

研究成果: 雜誌貢獻 › 文章

5 引文斯高帕斯（Scopus）

摘要

Phytohemagglutinin (PHA) stimulated lymphocytes were exposed to a hypotonic solution consisting of equal parts of 0.075 M 2-Mercaptoethanol and 0.075 M KCl, followed by addition of colcemid. The cells were fixed and washed in the usual manner. The slides then were subjected to trypsin-Giemsa banding. This method yields a high percentage of mitotic figures in prophase and prometaphase, ideal for high-resolution chromosome analysis.

原文	英語
頁（從 - 到）	481-483
頁數	3
期刊	American Journal of Clinical Pathology
卷	79
發行號	4
DOIs	https://doi.org/10.1093/ajcp/79.4.481
出版狀態	已發佈 - 一月 1 1983
對外發佈	Yes

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1093/ajcp/79.4.481

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引用此

Kao, Y. S., Whang-Peng, J., & Lee, E. (1983). A simple, rapid, high-resolution chromosome technic for lymphocytes. American Journal of Clinical Pathology, 79(4), 481-483. https://doi.org/10.1093/ajcp/79.4.481

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