A simple, rapid, high-resolution chromosome technic for lymphocytes

Y. S. Kao, J. Whang-Peng, E. Lee

研究成果: 雜誌貢獻文章

5 引文 斯高帕斯(Scopus)

摘要

Phytohemagglutinin (PHA) stimulated lymphocytes were exposed to a hypotonic solution consisting of equal parts of 0.075 M 2-Mercaptoethanol and 0.075 M KCl, followed by addition of colcemid. The cells were fixed and washed in the usual manner. The slides then were subjected to trypsin-Giemsa banding. This method yields a high percentage of mitotic figures in prophase and prometaphase, ideal for high-resolution chromosome analysis.
原文英語
頁(從 - 到)481-483
頁數3
期刊American Journal of Clinical Pathology
79
發行號4
DOIs
出版狀態已發佈 - 一月 1 1983
對外發佈Yes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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