A simple plant gene delivery system using mesoporous silica nanoparticles as carriers

Feng Peng Chang, Lin Yun Kuang, Chia An Huang, Wann Neng Jane, Yann Hung, Yue Ie C. Hsing, Chung Yuan Mou

研究成果: 雜誌貢獻文章同行評審

88 引文 斯高帕斯(Scopus)

摘要

A facile DNA delivery method would greatly facilitate studies of plant functional genomics. However, plant cell walls limit the utilization of nanoparticles on plant research. Here, we employed functionalized mesoporous silica nanoparticles (MSNs) to develop a MSN-mediated plant transient gene expression system. In this system, MSNs served as carriers to deliver foreign DNA into intact Arabidopsis thaliana roots without the aid of mechanical force. Gene expression was detected in the epidermal layer and in the more inner cortical and endodermal root tissues by both fluorescence and antibody labeling. This is a novel alternative to the conventional gene-gun or ultrasonic methods. In addition, the parameters that affect the MSN uptake and the mechanism and subcellular distribution of particles were also analyzed. The present study may provide valuable information on the manipulation of functional nanoparticles in plants and have significant impact on plant biotechnology.

原文英語
頁(從 - 到)5279-5287
頁數9
期刊Journal of Materials Chemistry B
1
發行號39
DOIs
出版狀態已發佈 - 10月 21 2013
對外發佈

ASJC Scopus subject areas

  • 化學 (全部)
  • 生物醫學工程
  • 材料科學(全部)

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