A seminal vesicle autoantigen of mouse is able to suppress sperm capacitation-related events stimulated by serum albumin

Y. H. Huang, S. T. Chu, Y. H. Chen

研究成果: 雜誌貢獻文章

43 引文 (Scopus)

摘要

We studied the effect of a mouse seminal vesicle autoantigen (SVA) on BSA-stimulated functions of mouse sperm. Uncapacitated, capacitated, and acrosome-reacted stages of sperm were morphologically scored, and the cellular zinc content was examined cytologically in a modified Tyrode solution at 37°C for 80 min. More than 85% of control cells remained uncapacitated. Addition of 0.3% SVA to the cell incubation did not affect the cell status. Approximately 65% of cells were capacitated in the incubation medium containing 0.3% BSA. Only 30% of the cells became capacitated after incubation with 0.3% BSA and 0.3% SVA together. The decapacitation effect by 0.3% SVA could be subdued by more than 3% BSA in the cell incubation. Whereas BSA did, SVA did not cause removal of Zn2+ from sperm, but SVA could suppress the BSA effect. The tyrosine phosphorylated proteins in sperm were detected after incubation in a modified HEPES medium containing 0.3% BSA and/or 0.3% SVA at 37°C for 90 min. Whereas BSA enhanced greatly, SVA did not cause phosphorylation of proteins in the range of M(r) 40 000-120 000. The BSA-stimulated protein tyrosine phosphorylation could be suppressed by SVA in the cell incubation.

原文英語
頁(從 - 到)1562-1566
頁數5
期刊Biology of Reproduction
63
發行號5
出版狀態已發佈 - 2000
對外發佈Yes

指紋

Sperm Capacitation
Serum Albumin
Spermatozoa
Tyrosine
Phosphorylation
HEPES
seminal vesicle autoantigen
Acrosome
Proteins
Zinc

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

引用此文

A seminal vesicle autoantigen of mouse is able to suppress sperm capacitation-related events stimulated by serum albumin. / Huang, Y. H.; Chu, S. T.; Chen, Y. H.

於: Biology of Reproduction, 卷 63, 編號 5, 2000, p. 1562-1566.

研究成果: 雜誌貢獻文章

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