A screening platform for compounds with potential immuno-regulatory activities using human cord blood mononuclear cells

Chia Jung Chen, Che Tsai Chia, Jung Feng Hsieh, Chichen Michael Chien, Tzu H. Wu, Shui Tein Chen

研究成果: 雜誌貢獻文章

6 引文 斯高帕斯(Scopus)

摘要

A systematic and combinatorial approach was adopted using human umbilical cord blood mononuclear cells (hUCB-MNCs) to screen for potential immuno-regulatory compounds. The hUCB-MNCs contain several types of immunogenic cells, which are a suitable material to mimic the in vivo immuno-response after drug treatment. hUCB-MNCs were treated with various natural products such as quercetin, astaxanthin, caffeic acid, bilobalide, eugenol, rutin and γ-dodecalactone (γ-DDL). Phenotypic expression analysis revealed that the subpopulation of CD3+ T cells, CD56+ NK cells and CD1a+ dendritic cells apparently increased after being treated with γ-DDL for 6 days. The expression of CD56 reached a maximum at 72 h with a dose-dependent relationship. The NK cells activation marker (CD69) also elevated following γ-DDL treatment. These results demonstrated that the γ-DDL has immuno-regulatory effects to enhance cord blood NK cells population and bioactivities. Such a high-throughput methodology using hUCB-MNCs may be an effective platform for systematically screening potential immuno-regulatory compounds.

原文英語
頁(從 - 到)777-784
頁數8
期刊Combinatorial Chemistry and High Throughput Screening
9
發行號10
DOIs
出版狀態已發佈 - 十二月 2006

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Chemistry (miscellaneous)
  • Pharmacology

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