A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis

Chien Han Kao, Chiu Min Cheng, Kuo Hsiang Chuang, Chih Hung Chuang, Shey Cherng Tzou, Ta Chun Cheng, Yuan Chin Hsieh, Kuang Wen Liao, Yun Ming Wang, Long Sen Chang, Steve R. Roffler, Fang Ming Chen, Tian Lu Cheng

研究成果: 雜誌貢獻文章同行評審

4 引文 斯高帕斯(Scopus)

摘要

We designed a protein ladder (hereafter referred to as "Mega- tag") that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder are highly correlated (R2= 0.997 ± 0.00232), indicating that the dye-free Mega-tag protein ladder is accurate. It can also serve as a positive control for anti-epitope tag immunoblots. The Mega-tag protein ladder should provide a convenient and precise tool for Western blot analysis.

原文英語
頁(從 - 到)1-3
頁數3
期刊Analytical Biochemistry
431
發行號1
DOIs
出版狀態已發佈 - 十二月 1 2012

ASJC Scopus subject areas

  • 生物物理學
  • 生物化學
  • 分子生物學
  • 細胞生物學

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