A reduced oxygen tension (5%) is not beneficial for maintaining human embryonic stem cells in the undifferentiated state with short splitting intervals

Hsin Fu Chen, Hung Chih Kuo, Wannhsin Chen, Fang Chun Wu, Yu Shih Yang, Hong Nerng Ho

研究成果: 雜誌貢獻文章同行評審

30 引文 斯高帕斯(Scopus)

摘要

BACKGROUND: Human embryos grow naturally in vivo in lower oxygen (O 2) tension environments than atmospheric O2 tension. Therefore, human embryonic stem cells (hESC), a derivative of embryos, will likely grow more favorably in a reduced O2 tension. This study aimed to compare the behavior of hESC under reduced O2 tension (5%) versus normoxia (21%). METHODS: hESC lines were cultured in different O2 tensions and then examined for morphology, apoptosis and gene expression profiles. RESULTS: hESC grown in 5% O2 tension were not morphologically different from hESC grown in normoxia on day 7 of the first and fourth passages. However, after prolonged culture without splitting (10-14 days), hESC colonies were thinner and looked better morphologically in 5% O 2, but the cells proliferated more slowly and their sizes were larger. At most time points, the gene expression profiles in both O2 tensions showed no major difference in representative stemness genes (Oct-3/4, Nanog and Cripto), differentiation genes (Desmin, Nestin, α-fetoprotein and GDF-9) and hypoxia-related genes (HIF-1α and VEGF). A lower level of cyclin-D1 mRNA (suggestive of less Wnt pathway signaling on day 7 of the fourth passage) and a higher level of Desmin (suggestive of more differentiation to mesoderm, at day 7 of the first passage) were detected in 5% O2. CONCLUSIONS: This study suggests that for routine culture of hESC with a short splitting interval (7 days), a low O2 tension (5% O2) probably does not provide significant advantages over the standard 21% O 2 tension for the maintenance of an undifferentiated state by the criteria used in this study.
原文英語
頁(從 - 到)71-80
頁數10
期刊Human Reproduction
24
發行號1
DOIs
出版狀態已發佈 - 一月 1 2009
對外發佈

ASJC Scopus subject areas

  • 生殖醫學
  • 婦產科

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