A preclinical evaluation of antrodia camphorata alcohol extracts in the treatment of non-small cell lung cancer using non-invasive molecular imaging

Jeng Fong Chiou, Alexander T H Wu, Wei Tin Wang, Tsu Hsiang Kuo, Juri G. Gelovani, I. Hsin Lin, Chih Hsiung Wu, Wen Ta Chiu, Win Ping Deng

研究成果: 雜誌貢獻文章

14 引文 斯高帕斯(Scopus)

摘要

This study was carried out to provide a platform for the pre-clinical evaluation of anti-cancer properties of a unique CAM (complementary and alternative medicine) agent, Antrodia camphorata alcohol extract (ACAE), in a mouse model with the advantageous non-invasive in vivo bioluminescence molecular imaging technology. In vitro analyses on the proliferation, migration/invasion, cell cycle and apoptosis were performed on ACAE-treated non-small cell lung cancer cells, H441GL and control CGL1 cells. In vivo, immune-deficient mice were inoculated subcutaneously with H441GL followed by oral gavages of ACAE. The effect of ACAE on tumor progression was monitored by non-invasive bioluminescence imaging. The proliferation and migration/invasion of H441GL cells were inhibited by ACAE in a dose-dependent manner. In addition, ACAE induced cell cycle arrest at G0/G1 phase and apoptosis in H441GL cells as shown by flow cytometric analysis, Annexin-V immunoflourescence and DNA fragmentation. In vivo bioluminescence imaging revealed that tumorigenesis was significantly retarded by oral treatment of ACAE in a dose-dependent fashion. Based on our experimental data, ACAE contains anti-cancer properties and could be considered as a potential CAM agent in future clinical evaluation.
原文英語
文章編號914561
期刊Evidence-based Complementary and Alternative Medicine
2011
DOIs
出版狀態已發佈 - 2011

ASJC Scopus subject areas

  • Complementary and alternative medicine

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