A preclinical evaluation of antrodia camphorata alcohol extracts in the treatment of non-small cell lung cancer using non-invasive molecular imaging

Jeng Fong Chiou, Alexander T H Wu, Wei Tin Wang, Tsu Hsiang Kuo, Juri G. Gelovani, I. Hsin Lin, Chih Hsiung Wu, Wen Ta Chiu, Win Ping Deng

研究成果: 雜誌貢獻文章

14 引文 (Scopus)

摘要

This study was carried out to provide a platform for the pre-clinical evaluation of anti-cancer properties of a unique CAM (complementary and alternative medicine) agent, Antrodia camphorata alcohol extract (ACAE), in a mouse model with the advantageous non-invasive in vivo bioluminescence molecular imaging technology. In vitro analyses on the proliferation, migration/invasion, cell cycle and apoptosis were performed on ACAE-treated non-small cell lung cancer cells, H441GL and control CGL1 cells. In vivo, immune-deficient mice were inoculated subcutaneously with H441GL followed by oral gavages of ACAE. The effect of ACAE on tumor progression was monitored by non-invasive bioluminescence imaging. The proliferation and migration/invasion of H441GL cells were inhibited by ACAE in a dose-dependent manner. In addition, ACAE induced cell cycle arrest at G0/G1 phase and apoptosis in H441GL cells as shown by flow cytometric analysis, Annexin-V immunoflourescence and DNA fragmentation. In vivo bioluminescence imaging revealed that tumorigenesis was significantly retarded by oral treatment of ACAE in a dose-dependent fashion. Based on our experimental data, ACAE contains anti-cancer properties and could be considered as a potential CAM agent in future clinical evaluation.
原文英語
文章編號914561
期刊Evidence-based Complementary and Alternative Medicine
2011
DOIs
出版狀態已發佈 - 2011

指紋

Antrodia
Molecular Imaging
Non-Small Cell Lung Carcinoma
Alcohols
Complementary Therapies
Therapeutics
Apoptosis
Neoplasms
Cell Cycle Resting Phase
Annexin A5
G1 Phase
DNA Fragmentation
Cell Cycle Checkpoints
Cell Cycle
Carcinogenesis
Technology

ASJC Scopus subject areas

  • Complementary and alternative medicine

引用此文

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title = "A preclinical evaluation of antrodia camphorata alcohol extracts in the treatment of non-small cell lung cancer using non-invasive molecular imaging",
abstract = "This study was carried out to provide a platform for the pre-clinical evaluation of anti-cancer properties of a unique CAM (complementary and alternative medicine) agent, Antrodia camphorata alcohol extract (ACAE), in a mouse model with the advantageous non-invasive in vivo bioluminescence molecular imaging technology. In vitro analyses on the proliferation, migration/invasion, cell cycle and apoptosis were performed on ACAE-treated non-small cell lung cancer cells, H441GL and control CGL1 cells. In vivo, immune-deficient mice were inoculated subcutaneously with H441GL followed by oral gavages of ACAE. The effect of ACAE on tumor progression was monitored by non-invasive bioluminescence imaging. The proliferation and migration/invasion of H441GL cells were inhibited by ACAE in a dose-dependent manner. In addition, ACAE induced cell cycle arrest at G0/G1 phase and apoptosis in H441GL cells as shown by flow cytometric analysis, Annexin-V immunoflourescence and DNA fragmentation. In vivo bioluminescence imaging revealed that tumorigenesis was significantly retarded by oral treatment of ACAE in a dose-dependent fashion. Based on our experimental data, ACAE contains anti-cancer properties and could be considered as a potential CAM agent in future clinical evaluation.",
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AU - Wu, Alexander T H

AU - Wang, Wei Tin

AU - Kuo, Tsu Hsiang

AU - Gelovani, Juri G.

AU - Lin, I. Hsin

AU - Wu, Chih Hsiung

AU - Chiu, Wen Ta

AU - Deng, Win Ping

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