A novel strategy for corneal endothelial reconstruction with a bioengineered cell sheet

Ging Ho Hsiue, Jui Yang Lai, Ko Hua Chen, Wen-Ming Hsu

研究成果: 雜誌貢獻文章同行評審

102 引文 斯高帕斯(Scopus)

摘要

Cellular organization of foreign grafts constructed from cultivated cells is critical to successful graft-host integration and tissue repair. This study described a novel human corneal endothelial cell (HCEC) therapeutic method, where cultivated adult HCEC sheet with uniform orientation was prepared and transplanted to a rabbit cornea. Having a correct morphology and intact barriers, the HCEC sheet was made by the temperature-modulated detachment of monolayered HCECs from thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm)-grafted surfaces and was delivered with proper polarity to the corneal posterior surface by a bioadhesive gelatin disc. Results of the in vivo studies, including the follow-up clinical observations and histological examinations, showed the laminated HCEC sheet was successfully integrated into rabbit cornea denuded with endothelial layer after the biodegradation of gelatin carrier. These data indicate the feasibility of the proposed procedure in cell therapy for corneal endothelial cell loss.

原文英語
頁(從 - 到)473-476
頁數4
期刊Transplantation
81
發行號3
DOIs
出版狀態已發佈 - 2月 1 2006
對外發佈

ASJC Scopus subject areas

  • 移植
  • 免疫學

指紋

深入研究「A novel strategy for corneal endothelial reconstruction with a bioengineered cell sheet」主題。共同形成了獨特的指紋。

引用此