A novel role for the klebsiella pneumoniae sap (sensitivity to antimicrobial peptides) transporter in intestinal cell interactions, innate immune responses, liver abscess, and virulence

Chun Ru Hsu, I. Wei Chang, Pei Fang Hsieh, Tzu Lung Lin, Pei Yin Liu, Chen Hsiu Huang, Kun Tzu Li, Jin Town Wang

研究成果: 雜誌貢獻文章同行評審

7 引文 斯高帕斯(Scopus)

摘要

Klebsiella pneumoniae is an important human pathogen causing hospital-acquired and community-acquired infections. Systemic K. pneumoniae infections may be preceded by gastrointestinal colonization, but the basis of this bacterium's interaction with the intestinal epithelium remains unclear. Here, we report that the K. pneumoniae Sap (sensitivity to antimicrobial peptides) transporter contributes to bacterial-host cell interactions and in vivo virulence. Gene deletion showed that sapA is required for the adherence of a K. pneumoniae blood isolate to intestinal epithelial, lung epithelial, urinary bladder epithelial, and liver cells. Te flsapA mutant was defcient for translocation across intestinal epithelial monolayers, macrophage interactions, and induction of proinflammatory cytokines. In a mouse gastrointestinal infection model, flsapA yielded signifcantly decreased bacterial loads in liver, spleen and intestine, reduced liver abscess generation, and decreased mortality. Tese fndings offer new insights into the pathogenic interaction of K. pneumoniae with the host gastrointestinal tract to cause systemic infection.

原文英語
頁(從 - 到)1294-1306
頁數13
期刊Journal of Infectious Diseases
219
發行號8
DOIs
出版狀態已發佈 - 四月 8 2019

ASJC Scopus subject areas

  • 免疫學和過敏
  • 傳染性疾病

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