A novel podophyllotoxin-derived compound GL331 is more potent than its congener VP-16 in killing refractory cancer cells

Tze Sing Huang, Chun Chung Lee, Yee Chao, Chih Hung Shu, Li Tzong Chen, Li Li Chen, Min Hsiung Chen, Chiou Chung Yuan, Jacqueline Whang-Peng

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60 引文 斯高帕斯(Scopus)

摘要

Purpose. GL331 is a new homolog of VP- 16, and has demonstrated more efficacious anti-cancer activity in both the in vitro and in vivo lymphoma systems. To extensively explore GL331 's clinical value, we furthermore evaluate the cytotoxicity and apoptosis-inducing activity of GL331 in several human cell lines from cancers that are not normally treated with VP-16. Methods. By MTT and clonogenic survival assays, the cytotoxicities of GL331 and VP-16 were evaluated in a variety of cell lines including nasopharyngeal, hepatocellular, gastric, colon, cervical, and neuroblastoma cancer types. Western blot analysis was performed to detect the MDR-1 level in these cell lines. By Annexin V-staining flow cytometry and detection of DNA ladders, the apoptosis-inducing activities of GL331 and VP-16 were also evaluated. Results. GL331 showed more efficacy than its congener VP-16 in killing cancer cells. The estimated ID50 of GL331 were 2.5 to 17-fold lower than those of VP-16. GL331 possessed more cell-killing activity even in MDR-1- overexpressing cell lines such as HCC36 and SW620. Its higher cytotoxicity could be attributed by the elevated ability to induce apoptotic cell death. Conclusion. GL331's overriding drug resistance and higher cancer cell-killing activity suggest its superiority in clinical cancer therapy.
原文英語
頁(從 - 到)997-1002
頁數6
期刊Pharmaceutical Research
16
發行號7
DOIs
出版狀態已發佈 - 一月 1 1999
對外發佈

ASJC Scopus subject areas

  • 生物技術
  • 分子醫學
  • 藥理
  • 藥學科學
  • 有機化學
  • 藥學(醫學)

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