A novel in vitro retinal differentiation model by co-culturing adult human bone marrow stem cells with retinal pigmented epithelium cells

Shih Hwa Chiou, Chung Lan Kao, Chi Hsien Peng, Shih Jen Chen, Yih Wen Tarng, Hung Hai Ku, Yu Chih Chen, Yi Ming Shyr, Ren Shyan Liu, Chien Jen Hsu, De Ming Yang, Wen-Ming Hsu, Cheng Deng Kuo, Chen Hsen Lee

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72 引文 斯高帕斯(Scopus)

摘要

Human retinal pigment epithelium (HRPE) cells are important in maintaining the normal physiology within the neurosensory retina and photoreceptors. Recently, transplantation of HRPE has become a possible therapeutic approach for retinal degeneration. By negative immunoselection (CD45 and glycophorin A), in this study, we have isolated and cultivated adult human bone marrow stem cells (BMSCs) with multilineage differentiation potential. After a 2- to 4-week culture under chondrogenic, osteogenic, adipogenic, and hepatogenic induction medium, these BMSCs were found to differentiate into cartilage, bone, adipocyte, and hepatocyte-like cells, respectively. We also showed that these BMSCs could differentiate into neural precursor cells (nestin-positive) and mature neurons (MAP-2 and Tuj1-positive) following treatment of neural selection and induction medium for 1 month. Furthermore, the plasticity of BMSCs was confirmed by initiating their differentiation into retinal cells and photoreceptor lineages by co-culturing with HRPE cells. The latter system provides an ex vivo expansion model of culturing photoreceptors for the treatment of retinal degeneration diseases.

原文英語
頁(從 - 到)578-585
頁數8
期刊Biochemical and Biophysical Research Communications
326
發行號3
DOIs
出版狀態已發佈 - 1月 21 2005
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 生物化學
  • 分子生物學
  • 細胞生物學

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