A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid

Kuan Chung Chen, Ching Yu Lin, Cheng Chun Kuan, Hsien Yi Sung, Ching San Chen

研究成果: 雜誌貢獻文章

73 引文 斯高帕斯(Scopus)

摘要

A cDNA encoding a small cysteine-rich protein designated VrCRP was isolated from a bruchid-resistant mungbean. VrCRP encodes a protein of 73 amino acids containing a 27 amino acid signal peptide and 8 cysteines. On the basis of the amino acid sequence similarity and conserved residues, it is suggested that VrCRP is a member of the plant defensin family. VrCRP protein was obtained by overexpression of VrCRP with a truncated signal peptide in an IMPACT system. Artificial seeds containing 0.2% (w/w) of the purified VrCRP-TSP were lethal to larvae of the bruchid Callosobruchus chinensis. VrCRP is apparently the first reported plant defensin exhibiting in vitro insecticidal activity against C. chinensis.
原文英語
頁(從 - 到)7258-7263
頁數6
期刊Journal of Agricultural and Food Chemistry
50
發行號25
DOIs
出版狀態已發佈 - 十二月 4 2002
對外發佈Yes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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