A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid

Kuan Chung Chen; Ching Yu Lin; Cheng Chun Kuan; Hsien Yi Sung; Ching San Chen

doi:10.1021/jf020527q

A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid

Kuan Chung Chen, Ching Yu Lin, Cheng Chun Kuan, Hsien Yi Sung, Ching San Chen

研究成果: 雜誌貢獻 › 文章 › 同行評審

78 引文斯高帕斯（Scopus）

摘要

A cDNA encoding a small cysteine-rich protein designated VrCRP was isolated from a bruchid-resistant mungbean. VrCRP encodes a protein of 73 amino acids containing a 27 amino acid signal peptide and 8 cysteines. On the basis of the amino acid sequence similarity and conserved residues, it is suggested that VrCRP is a member of the plant defensin family. VrCRP protein was obtained by overexpression of VrCRP with a truncated signal peptide in an IMPACT system. Artificial seeds containing 0.2% (w/w) of the purified VrCRP-TSP were lethal to larvae of the bruchid Callosobruchus chinensis. VrCRP is apparently the first reported plant defensin exhibiting in vitro insecticidal activity against C. chinensis.

原文	英語
頁（從 - 到）	7258-7263
頁數	6
期刊	Journal of Agricultural and Food Chemistry
卷	50
發行號	25
DOIs	https://doi.org/10.1021/jf020527q
出版狀態	已發佈 - 十二月 4 2002
對外發佈	是

ASJC Scopus subject areas

農業與生物科學（雜項）
食品科學
化學（雜項）

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10.1021/jf020527q

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abstract = "A cDNA encoding a small cysteine-rich protein designated VrCRP was isolated from a bruchid-resistant mungbean. VrCRP encodes a protein of 73 amino acids containing a 27 amino acid signal peptide and 8 cysteines. On the basis of the amino acid sequence similarity and conserved residues, it is suggested that VrCRP is a member of the plant defensin family. VrCRP protein was obtained by overexpression of VrCRP with a truncated signal peptide in an IMPACT system. Artificial seeds containing 0.2% (w/w) of the purified VrCRP-TSP were lethal to larvae of the bruchid Callosobruchus chinensis. VrCRP is apparently the first reported plant defensin exhibiting in vitro insecticidal activity against C. chinensis.",

keywords = "Bruchid resistance, Defensin, In vitro translation, Mungbean",

author = "Chen, {Kuan Chung} and Lin, {Ching Yu} and Kuan, {Cheng Chun} and Sung, {Hsien Yi} and Chen, {Ching San}",

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AU - Chen, Ching San

PY - 2002/12/4

Y1 - 2002/12/4

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KW - In vitro translation

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A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid

摘要

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