A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid

Kuan Chung Chen, Ching Yu Lin, Cheng Chun Kuan, Hsien Yi Sung, Ching San Chen

研究成果: 雜誌貢獻 › 文章

72 引文 (Scopus)

摘要

A cDNA encoding a small cysteine-rich protein designated VrCRP was isolated from a bruchid-resistant mungbean. VrCRP encodes a protein of 73 amino acids containing a 27 amino acid signal peptide and 8 cysteines. On the basis of the amino acid sequence similarity and conserved residues, it is suggested that VrCRP is a member of the plant defensin family. VrCRP protein was obtained by overexpression of VrCRP with a truncated signal peptide in an IMPACT system. Artificial seeds containing 0.2% (w/w) of the purified VrCRP-TSP were lethal to larvae of the bruchid Callosobruchus chinensis. VrCRP is apparently the first reported plant defensin exhibiting in vitro insecticidal activity against C. chinensis.
原文英語
頁（從 - 到）7258-7263
頁數6
期刊Journal of Agricultural and Food Chemistry
卷50
發行號25
DOIs
出版狀態已發佈 - 十二月 4 2002
對外發佈Yes

指紋

Defensins
mung beans
Callosobruchus chinensis
Complementary DNA
Protein Sorting Signals
signal peptide
Amino Acids
Cysteine
cysteine
artificial seeds
Proteins
amino acids
proteins
lethal genes
Larva
Seed
Amino Acid Sequence
Seeds
amino acid sequences
larvae

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

引用此文

A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid. / Chen, Kuan Chung; Lin, Ching Yu; Kuan, Cheng Chun; Sung, Hsien Yi; Chen, Ching San.

於: Journal of Agricultural and Food Chemistry, 卷 50, 編號 25, 04.12.2002, p. 7258-7263.

研究成果: 雜誌貢獻 › 文章

Chen, KC, Lin, CY, Kuan, CC, Sung, HY & Chen, CS 2002, 'A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid', Journal of Agricultural and Food Chemistry, 卷 50, 編號 25, 頁 7258-7263. https://doi.org/10.1021/jf020527q
Chen KC, Lin CY, Kuan CC, Sung HY, Chen CS. A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid. Journal of Agricultural and Food Chemistry. 2002 12月 4;50(25):7258-7263. https://doi.org/10.1021/jf020527q
Chen, Kuan Chung ; Lin, Ching Yu ; Kuan, Cheng Chun ; Sung, Hsien Yi ; Chen, Ching San. / A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid. 於: Journal of Agricultural and Food Chemistry. 2002 ; 卷 50, 編號 25. 頁 7258-7263.
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