摘要
A cDNA encoding a small cysteine-rich protein designated VrCRP was isolated from a bruchid-resistant mungbean. VrCRP encodes a protein of 73 amino acids containing a 27 amino acid signal peptide and 8 cysteines. On the basis of the amino acid sequence similarity and conserved residues, it is suggested that VrCRP is a member of the plant defensin family. VrCRP protein was obtained by overexpression of VrCRP with a truncated signal peptide in an IMPACT system. Artificial seeds containing 0.2% (w/w) of the purified VrCRP-TSP were lethal to larvae of the bruchid Callosobruchus chinensis. VrCRP is apparently the first reported plant defensin exhibiting in vitro insecticidal activity against C. chinensis.
原文 | 英語 |
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頁(從 - 到) | 7258-7263 |
頁數 | 6 |
期刊 | Journal of Agricultural and Food Chemistry |
卷 | 50 |
發行號 | 25 |
DOIs | |
出版狀態 | 已發佈 - 12月 4 2002 |
對外發佈 | 是 |
ASJC Scopus subject areas
- 農業與生物科學(雜項)
- 食品科學
- 化學(雜項)