A novel core fractionation process of human plasma by expanded bed adsorption chromatography

Allan Lihme, Marie Bendix Hansen, Inga Vaarst Andersen, Thierry Burnouf

研究成果: 雜誌貢獻文章同行評審

26 引文 斯高帕斯(Scopus)

摘要

Current plasma fractionation technology combines ethanol precipitation with packed bed chromatography. We have developed a novel core fractionation process comprising five expanded bed adsorption (EBA) chromatographic steps on high-density modified agarose/tungsten carbide beads. Plasma was first chromatographed on two diethyl amino-ethyl (DEAE)-tungsten carbide agarose adsorbents (respective mean particle diameters of dv(0.5)=190 and 37μm) to isolate at 50 to 80% recovery a fraction containing 4 to 7IU/ml factor II (FII), factor IX (FIX), and factor X (FX) (specific activity >1IU/mg) and another enriched in FVIII and von Willebrand factor (vWF) (∼1IU/ml and 0.6IU/mg, respectively). The flow-through was adsorbed on 4% agarose-10% tungsten carbide beads coupled with an acidic mixed-mode ligand to isolate an 80% pure immunoglobulin G (IgG) at a 93% step recovery. A highly purified α1-antitrypsin was isolated at 95% step recovery by adsorbing the flow-through on 4% epoxy-crosslinked agarose-10% tungsten carbide adsorbent material coupled with a cationic ligand. Isolation of 98% pure albumin was achieved at a 99% step recovery by pH 4.5 adsorption of the flow-through on 6% agarose-10% tungsten carbide beads coupled with an acidic mixed-mode ligand. EBA may represent a feasible alternative core plasma fractionation tool.
原文英語
頁(從 - 到)102-109
頁數8
期刊Analytical Biochemistry
399
發行號1
DOIs
出版狀態已發佈 - 4月 2010
對外發佈

ASJC Scopus subject areas

  • 生物化學
  • 生物物理學
  • 細胞生物學
  • 分子生物學

指紋

深入研究「A novel core fractionation process of human plasma by expanded bed adsorption chromatography」主題。共同形成了獨特的指紋。

引用此