A new approach to facilitate diagnosis of nonalcoholic fatty liver disease through a galactose single point method in rats with fatty liver

Jr Ting Lee, Li Heng Pao, Meei Shyuan Lee, Jiunn Wang Liao, Chi Min Shih, Cheng Huei Hsiong, Fon Yi Yin, Tung Yuan Shih, Oliver Yoa Pu Hu

研究成果: 雜誌貢獻文章

2 引文 (Scopus)

摘要

Background: Liver biopsy reliably diagnoses nonalcoholic fatty liver disease, but its invasiveness and inter- and intra-observer errors limit its usefulness in monitoring. Aims: Use a galactose single point method or combined biochemical parameters to improve assessments of nonalcoholic fatty liver disease in a rat model. Methods: Three nonalcoholic fatty liver disease severities were generated in 50 rats: a control group (n= 18) on a standard diet, and 2 study groups on a choline-deficient diet (n= 18), with and without treatment with silymarin (n= 14). At weeks 4, 8, and 18, a galactose solution (0.5. g/kg/body weight) was rapidly injected intravenously. Sixty minutes later, internal artery blood was taken for biochemical analyses, including galactose. The livers were then removed for haematoxylin-eosin staining and to measure the hepatic lipid content. Results: Alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, albumin, and total protein were each significantly correlated with nonalcoholic fatty liver disease severity. Regarding logistic regression, galactose single point method and total protein were significantly predictive. The optimal alanine aminotransferase cutoff point for nonalcoholic fatty liver disease prediction from the receiver-operating characteristic curve had 72.4% sensitivity and 52.4% specificity; galactose single point method alone had 82.8% and 72.4%, whereas galactose single point method. +. total protein showed 82.8% and 81.0%. Conclusions: Both galactose single point method and galactose single point method. +. total protein had greater diagnostic sensitivity and specificity for nonalcoholic fatty liver disease than traditional biochemical tests.
原文英語
頁(從 - 到)134-141
頁數8
期刊Digestive and Liver Disease
45
發行號2
DOIs
出版狀態已發佈 - 二月 1 2013
對外發佈Yes

指紋

Fatty Liver
Galactose
Alanine Transaminase
Liver
Proteins
Silymarin
Diet
Sensitivity and Specificity
Non-alcoholic Fatty Liver Disease
Hematoxylin
Eosine Yellowish-(YS)
Aspartate Aminotransferases
Choline
ROC Curve
Alkaline Phosphatase
Albumins
Arteries
Logistic Models
Body Weight
Staining and Labeling

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

引用此文

A new approach to facilitate diagnosis of nonalcoholic fatty liver disease through a galactose single point method in rats with fatty liver. / Lee, Jr Ting; Pao, Li Heng; Lee, Meei Shyuan; Liao, Jiunn Wang; Shih, Chi Min; Hsiong, Cheng Huei; Yin, Fon Yi; Shih, Tung Yuan; Hu, Oliver Yoa Pu.

於: Digestive and Liver Disease, 卷 45, 編號 2, 01.02.2013, p. 134-141.

研究成果: 雜誌貢獻文章

Lee, Jr Ting ; Pao, Li Heng ; Lee, Meei Shyuan ; Liao, Jiunn Wang ; Shih, Chi Min ; Hsiong, Cheng Huei ; Yin, Fon Yi ; Shih, Tung Yuan ; Hu, Oliver Yoa Pu. / A new approach to facilitate diagnosis of nonalcoholic fatty liver disease through a galactose single point method in rats with fatty liver. 於: Digestive and Liver Disease. 2013 ; 卷 45, 編號 2. 頁 134-141.
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title = "A new approach to facilitate diagnosis of nonalcoholic fatty liver disease through a galactose single point method in rats with fatty liver",
abstract = "Background: Liver biopsy reliably diagnoses nonalcoholic fatty liver disease, but its invasiveness and inter- and intra-observer errors limit its usefulness in monitoring. Aims: Use a galactose single point method or combined biochemical parameters to improve assessments of nonalcoholic fatty liver disease in a rat model. Methods: Three nonalcoholic fatty liver disease severities were generated in 50 rats: a control group (n= 18) on a standard diet, and 2 study groups on a choline-deficient diet (n= 18), with and without treatment with silymarin (n= 14). At weeks 4, 8, and 18, a galactose solution (0.5. g/kg/body weight) was rapidly injected intravenously. Sixty minutes later, internal artery blood was taken for biochemical analyses, including galactose. The livers were then removed for haematoxylin-eosin staining and to measure the hepatic lipid content. Results: Alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, albumin, and total protein were each significantly correlated with nonalcoholic fatty liver disease severity. Regarding logistic regression, galactose single point method and total protein were significantly predictive. The optimal alanine aminotransferase cutoff point for nonalcoholic fatty liver disease prediction from the receiver-operating characteristic curve had 72.4{\%} sensitivity and 52.4{\%} specificity; galactose single point method alone had 82.8{\%} and 72.4{\%}, whereas galactose single point method. +. total protein showed 82.8{\%} and 81.0{\%}. Conclusions: Both galactose single point method and galactose single point method. +. total protein had greater diagnostic sensitivity and specificity for nonalcoholic fatty liver disease than traditional biochemical tests.",
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author = "Lee, {Jr Ting} and Pao, {Li Heng} and Lee, {Meei Shyuan} and Liao, {Jiunn Wang} and Shih, {Chi Min} and Hsiong, {Cheng Huei} and Yin, {Fon Yi} and Shih, {Tung Yuan} and Hu, {Oliver Yoa Pu}",
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T1 - A new approach to facilitate diagnosis of nonalcoholic fatty liver disease through a galactose single point method in rats with fatty liver

AU - Lee, Jr Ting

AU - Pao, Li Heng

AU - Lee, Meei Shyuan

AU - Liao, Jiunn Wang

AU - Shih, Chi Min

AU - Hsiong, Cheng Huei

AU - Yin, Fon Yi

AU - Shih, Tung Yuan

AU - Hu, Oliver Yoa Pu

PY - 2013/2/1

Y1 - 2013/2/1

N2 - Background: Liver biopsy reliably diagnoses nonalcoholic fatty liver disease, but its invasiveness and inter- and intra-observer errors limit its usefulness in monitoring. Aims: Use a galactose single point method or combined biochemical parameters to improve assessments of nonalcoholic fatty liver disease in a rat model. Methods: Three nonalcoholic fatty liver disease severities were generated in 50 rats: a control group (n= 18) on a standard diet, and 2 study groups on a choline-deficient diet (n= 18), with and without treatment with silymarin (n= 14). At weeks 4, 8, and 18, a galactose solution (0.5. g/kg/body weight) was rapidly injected intravenously. Sixty minutes later, internal artery blood was taken for biochemical analyses, including galactose. The livers were then removed for haematoxylin-eosin staining and to measure the hepatic lipid content. Results: Alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, albumin, and total protein were each significantly correlated with nonalcoholic fatty liver disease severity. Regarding logistic regression, galactose single point method and total protein were significantly predictive. The optimal alanine aminotransferase cutoff point for nonalcoholic fatty liver disease prediction from the receiver-operating characteristic curve had 72.4% sensitivity and 52.4% specificity; galactose single point method alone had 82.8% and 72.4%, whereas galactose single point method. +. total protein showed 82.8% and 81.0%. Conclusions: Both galactose single point method and galactose single point method. +. total protein had greater diagnostic sensitivity and specificity for nonalcoholic fatty liver disease than traditional biochemical tests.

AB - Background: Liver biopsy reliably diagnoses nonalcoholic fatty liver disease, but its invasiveness and inter- and intra-observer errors limit its usefulness in monitoring. Aims: Use a galactose single point method or combined biochemical parameters to improve assessments of nonalcoholic fatty liver disease in a rat model. Methods: Three nonalcoholic fatty liver disease severities were generated in 50 rats: a control group (n= 18) on a standard diet, and 2 study groups on a choline-deficient diet (n= 18), with and without treatment with silymarin (n= 14). At weeks 4, 8, and 18, a galactose solution (0.5. g/kg/body weight) was rapidly injected intravenously. Sixty minutes later, internal artery blood was taken for biochemical analyses, including galactose. The livers were then removed for haematoxylin-eosin staining and to measure the hepatic lipid content. Results: Alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, albumin, and total protein were each significantly correlated with nonalcoholic fatty liver disease severity. Regarding logistic regression, galactose single point method and total protein were significantly predictive. The optimal alanine aminotransferase cutoff point for nonalcoholic fatty liver disease prediction from the receiver-operating characteristic curve had 72.4% sensitivity and 52.4% specificity; galactose single point method alone had 82.8% and 72.4%, whereas galactose single point method. +. total protein showed 82.8% and 81.0%. Conclusions: Both galactose single point method and galactose single point method. +. total protein had greater diagnostic sensitivity and specificity for nonalcoholic fatty liver disease than traditional biochemical tests.

KW - Assessing liver function

KW - Choline-deficient diet

KW - Galactose single point method (GSP)

KW - Nonalcoholic fatty liver disease (NAFLD)

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DO - 10.1016/j.dld.2012.08.019

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