A multiplexed quantitative strategy for membrane proteomics: Opportunities for mining therapeutic targets for autosomal dominant polycystic kidney disease

Chia Li Han, Chih Wei Chien, Wen Cheng Chen, Yet Ran Chen, Chien Peng Wu, Hung Li, Yu Ju Chen

研究成果: 雜誌貢獻文章同行評審

124 引文 斯高帕斯(Scopus)

摘要

Toward multiplexed, comprehensive, and robust quantitation of the membrane proteome, we report a strategy combining gel-assisted digestion, iTRAQ (isobaric tags for relative and absolute quantitation) labeling, and LCMS/MS. Quantitation of four independently purified membrane fractions from HeLa cells gave high accuracy (

原文英語
頁(從 - 到)1983-1997
頁數15
期刊Molecular and Cellular Proteomics
7
發行號10
DOIs
出版狀態已發佈 - 十月 2008
對外發佈Yes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

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