A monounsaturated fatty acid (oleic acid) modulates electrical activity in atrial myocytes with calcium and sodium dysregulation

Yung Kuo Lin, Yao Chang Chen, Yu Hsun Kao, Chin Feng Tsai, Yung Hsin Yeh, Jin Long Huang, Chen Chuan Cheng, Shih Ann Chen, Yi Jen Chen

研究成果: 雜誌貢獻文章同行評審

9 引文 斯高帕斯(Scopus)

摘要

Background Obesity and metabolic syndrome are important risk factors for atrial fibrillation. High plasma concentrations of monounsaturated fatty acids, including oleic acid (OLA), are frequently noted in obese individuals and patients with metabolic syndrome. However, it is not clear whether monounsaturated fatty acids (MUFAs) can directly modulate the electrophysiological characteristics of atrial myocytes. Methods Whole-cell patch clamp, indo-1 fluorescence, and Western blot analyses were used to record the action potentials (APs), ionic currents, and protein expressions of HL-1 myocytes incubated with and without (control) OLA (0.5 mM) for 24 h. Results Compared to control myocytes (n = 14), OLA-treated myocytes (n = 16) had shorter APD90 (65 ± 6 vs. 85 ± 6 ms, p <0.05) and APD 50 (24 ± 6 vs. 38 ± 4 ms, p <0.05) with a higher incidence of delayed afterdepolarizations (35.7% vs. 7%, p <0.05), which were suppressed by 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS, a blocker of the calcium-activated chloride current). In addition, OLA-treated myocytes (n = 19) exhibited larger calcium transients (0.54 ± 0.06 vs. 0.38 ± 0.05 R410/485, p <0.05), and sarcoplasmic reticular calcium contents (0.91 ± 0.05 vs. 0.64 ± 0.08 R410/485, p <0.05) than control myocytes (n = 15). OLA-treated myocytes had larger late sodium currents, smaller sodium-calcium exchanger currents, and smaller sodium-potassium pump currents. Moreover OLA-treated myocytes had higher expressions of sarcoplasmic reticular Ca2 +-ATPase and calmodulin kinase II, but lower expression of the sodium-potassium ATPase protein than control myocytes. Conclusions MUFAs can regulate atrial electrophysiological characteristics with calcium and sodium dysregulation, which may contribute to atrial arrhythmogenesis.

原文英語
頁(從 - 到)191-198
頁數8
期刊International Journal of Cardiology
176
發行號1
DOIs
出版狀態已發佈 - 2014

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Medicine(all)

指紋 深入研究「A monounsaturated fatty acid (oleic acid) modulates electrical activity in atrial myocytes with calcium and sodium dysregulation」主題。共同形成了獨特的指紋。

引用此