A modifier screen for bazooka/PAR-3 interacting genes in the Drosophila embryo epithelium

Wei Shao, Johnny Wu, Jeyla Chen, Donghoon M. Lee, Alisa Tishkina, Tony J.C. Harris

研究成果: 雜誌貢獻文章

17 引文 (Scopus)

摘要

Background: The development and homeostasis of multicellular organisms depends on sheets of epithelial cells. Bazooka (Baz; PAR-3) localizes to the apical circumference of epithelial cells and is a key hub in the protein interaction network regulating epithelial structure. We sought to identify additional proteins that function with Baz to regulate epithelial structure in the Drosophila embryo. Methodology/Principal Findings: The baz zygotic mutant cuticle phenotype could be dominantly enhanced by loss of known interaction partners. To identify additional enhancers, we screened molecularly defined chromosome 2 and 3 deficiencies. 37 deficiencies acted as strong dominant enhancers. Using deficiency mapping, bioinformatics, and available single gene mutations, we identified 17 interacting genes encoding known and predicted polarity, cytoskeletal, transmembrane, trafficking and signaling proteins. For each gene, their loss of function enhanced adherens junction defects in zygotic baz mutants during early embryogenesis. To further evaluate involvement in epithelial polarity, we generated GFP fusion proteins for 15 of the genes which had not been found to localize to the apical domain previously. We found that GFP fusion proteins for Drosophila ASAP, Arf79F, CG11210, Septin 5 and Sds22 could be recruited to the apical circumference of epithelial cells. Nine of the other proteins showed various intracellular distributions, and one was not detected. Conclusions/Significance: Our enhancer screen identified 17 genes that function with Baz to regulate epithelial structure in the Drosophila embryo. Our secondary localization screen indicated that some of the proteins may affect epithelial cell polarity by acting at the apical cell cortex while others may act through intracellular processes. For 13 of the 17 genes, this is the first report of a link to baz or the regulation of epithelial structure.
原文英語
文章編號e9938
期刊PLoS One
5
發行號4
DOIs
出版狀態已發佈 - 九月 10 2010
對外發佈Yes

指紋

modifiers (genes)
Drosophila
embryo (animal)
epithelium
Embryonic Structures
Epithelium
Genes
Epithelial Cells
epithelial cells
Proteins
genes
proteins
Septins
Protein Interaction Maps
Fusion reactions
Adherens Junctions
Cell Polarity
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 2
Protein Transport

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

引用此文

A modifier screen for bazooka/PAR-3 interacting genes in the Drosophila embryo epithelium. / Shao, Wei; Wu, Johnny; Chen, Jeyla; Lee, Donghoon M.; Tishkina, Alisa; Harris, Tony J.C.

於: PLoS One, 卷 5, 編號 4, e9938, 10.09.2010.

研究成果: 雜誌貢獻文章

Shao, Wei ; Wu, Johnny ; Chen, Jeyla ; Lee, Donghoon M. ; Tishkina, Alisa ; Harris, Tony J.C. / A modifier screen for bazooka/PAR-3 interacting genes in the Drosophila embryo epithelium. 於: PLoS One. 2010 ; 卷 5, 編號 4.
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