A modified protein precipitation procedure for efficient removal of albumin from serum

Yi Yun Chen, Shu Yu Lin, Yuh Ying Yeh, He Hsuan Hsiao, Chi Yue Wu, Shui Tsung Chen, Andrew H.J. Wang

研究成果: 雜誌貢獻文章同行評審

154 引文 斯高帕斯(Scopus)

摘要

Proteomic analysis of sera and the quest for identifying serum proteins as disease markers have often been hampered by the predominance of several highly abundant proteins including albumin and immunoglobulins. Prior albumin depletion so as to enrich for otherwise undetectable serum components is therefore a prerequisite in mining the serum proteome. In the course of evaluating several available methods and commercial kits, we have been able to refine the albumin depletion protocols and establish a modified albumin removal method using trichloroacetic acid (TCA)/acetone. Changes in major protein bands were monitored by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1-D SDS-PAGE) and used as the first screening strategy to evaluate and optimize for the precipitation experimental conditions. Our method showed better performance in efficiency, specificity, and costs in comparison with two commercially available albumin removal kits, and provides a simple prefractionation step for the proteomic analysis of serum biomarkers. Albumin isolated by the modified method is in the native state. Our method may offer a rapid method for purifying serum albumin in large scale.

原文英語
頁(從 - 到)2117-2127
頁數11
期刊Electrophoresis
26
發行號11
DOIs
出版狀態已發佈 - 6月 2005
對外發佈

ASJC Scopus subject areas

  • 分析化學
  • 生物化學
  • 臨床生物化學

指紋

深入研究「A modified protein precipitation procedure for efficient removal of albumin from serum」主題。共同形成了獨特的指紋。

引用此