摘要
Background: Vascular or nasal mucosal strips have been successfully used to study smooth muscle contractility in vitro. Using the trachea of the rat, we developed a simple and rapid in vitro technique for testing the effects of drugs that induce tracheal constriction or relaxation. Methods: We used our preparation to test the effectiveness of acetylcholine and methacholine as tracheal contraction drugs and the effectiveness of verapamil as a tracheal relaxing drug. A 5 mm long portion of rat trachea was submersed in 30 ml Kreb's solution in a muscle bath at room temperature. Changes in tracheal contractility in response to the application of parasympathetic mimetic agents were measured using a transducer connected to a Pentium III computer equipped with polygraphy software. Results: Addition of parasympathetic mimetics to the incubation medium caused the tracheal to contract in a dose-dependent manner. Addition of verapamil, a relaxation agent, induced a relaxation response only when the preparation had been pretreated with a tracheal constricting agent such as methacholine. Responses to verapamil were dose-dependent. Conclusion: The degree of drug-induced tracheal contraction or relaxation is dose-dependent. This method could prove useful for studying the effects of drugs on tracheal smooth muscle activity.
| 原文 | 英語 |
|---|---|
| 頁（從 - 到） | 203-206 |
| 頁數 | 4 |
| 期刊 | Journal of Medical Sciences (Taiwan) |
| 卷 | 27 |
| 發行號 | 5 |
| 出版狀態 | 已發佈 - 十月 2007 |
| 對外發佈 | Yes |
指紋
ASJC Scopus subject areas
- Medicine(all)
引用此文
A modified in vitro method for studying tracheal smooth muscle response to drugs. / Wang, Hsing Won; Wang, Yueh Ting; Wu, Chi Chung.
於: Journal of Medical Sciences (Taiwan), 卷 27, 編號 5, 10.2007, p. 203-206.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - A modified in vitro method for studying tracheal smooth muscle response to drugs
AU - Wang, Hsing Won
AU - Wang, Yueh Ting
AU - Wu, Chi Chung
PY - 2007/10
Y1 - 2007/10
N2 - Background: Vascular or nasal mucosal strips have been successfully used to study smooth muscle contractility in vitro. Using the trachea of the rat, we developed a simple and rapid in vitro technique for testing the effects of drugs that induce tracheal constriction or relaxation. Methods: We used our preparation to test the effectiveness of acetylcholine and methacholine as tracheal contraction drugs and the effectiveness of verapamil as a tracheal relaxing drug. A 5 mm long portion of rat trachea was submersed in 30 ml Kreb's solution in a muscle bath at room temperature. Changes in tracheal contractility in response to the application of parasympathetic mimetic agents were measured using a transducer connected to a Pentium III computer equipped with polygraphy software. Results: Addition of parasympathetic mimetics to the incubation medium caused the tracheal to contract in a dose-dependent manner. Addition of verapamil, a relaxation agent, induced a relaxation response only when the preparation had been pretreated with a tracheal constricting agent such as methacholine. Responses to verapamil were dose-dependent. Conclusion: The degree of drug-induced tracheal contraction or relaxation is dose-dependent. This method could prove useful for studying the effects of drugs on tracheal smooth muscle activity.
AB - Background: Vascular or nasal mucosal strips have been successfully used to study smooth muscle contractility in vitro. Using the trachea of the rat, we developed a simple and rapid in vitro technique for testing the effects of drugs that induce tracheal constriction or relaxation. Methods: We used our preparation to test the effectiveness of acetylcholine and methacholine as tracheal contraction drugs and the effectiveness of verapamil as a tracheal relaxing drug. A 5 mm long portion of rat trachea was submersed in 30 ml Kreb's solution in a muscle bath at room temperature. Changes in tracheal contractility in response to the application of parasympathetic mimetic agents were measured using a transducer connected to a Pentium III computer equipped with polygraphy software. Results: Addition of parasympathetic mimetics to the incubation medium caused the tracheal to contract in a dose-dependent manner. Addition of verapamil, a relaxation agent, induced a relaxation response only when the preparation had been pretreated with a tracheal constricting agent such as methacholine. Responses to verapamil were dose-dependent. Conclusion: The degree of drug-induced tracheal contraction or relaxation is dose-dependent. This method could prove useful for studying the effects of drugs on tracheal smooth muscle activity.
KW - In vitro study
KW - Smooth muscle
KW - Trachea
UR - http://www.scopus.com/inward/record.url?scp=35948980367&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=35948980367&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:35948980367
VL - 27
SP - 203
EP - 206
JO - Journal of Medical Sciences
JF - Journal of Medical Sciences
SN - 1011-4564
IS - 5
ER -