A modified in vitro method for studying tracheal smooth muscle response to drugs

Hsing Won Wang, Yueh Ting Wang, Chi Chung Wu

研究成果: 雜誌貢獻文章同行評審

12 引文 斯高帕斯(Scopus)


Background: Vascular or nasal mucosal strips have been successfully used to study smooth muscle contractility in vitro. Using the trachea of the rat, we developed a simple and rapid in vitro technique for testing the effects of drugs that induce tracheal constriction or relaxation. Methods: We used our preparation to test the effectiveness of acetylcholine and methacholine as tracheal contraction drugs and the effectiveness of verapamil as a tracheal relaxing drug. A 5 mm long portion of rat trachea was submersed in 30 ml Kreb's solution in a muscle bath at room temperature. Changes in tracheal contractility in response to the application of parasympathetic mimetic agents were measured using a transducer connected to a Pentium III computer equipped with polygraphy software. Results: Addition of parasympathetic mimetics to the incubation medium caused the tracheal to contract in a dose-dependent manner. Addition of verapamil, a relaxation agent, induced a relaxation response only when the preparation had been pretreated with a tracheal constricting agent such as methacholine. Responses to verapamil were dose-dependent. Conclusion: The degree of drug-induced tracheal contraction or relaxation is dose-dependent. This method could prove useful for studying the effects of drugs on tracheal smooth muscle activity.

頁(從 - 到)203-206
期刊Journal of Medical Sciences (Taiwan)
出版狀態已發佈 - 10月 2007

ASJC Scopus subject areas

  • 醫藥 (全部)


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