A human-specific switch of alternatively spliced AFMID isoforms contributes to TP53 mutations and tumor recurrence in hepatocellular carcinoma

Kuan Ting Lin, Wai Kit Ma, Juergen Scharner, Yun Ru Liu, Adrian R. Krainer

研究成果: 雜誌貢獻文章

3 引文 斯高帕斯(Scopus)

摘要

Pre-mRNA splicing can contribute to the switch of cell identity that occurs in carcinogenesis. Here, we analyze a large collection of RNA-seq data sets and report that splicing changes in hepatocyte-specific enzymes, such as AFMID and KHK, are associated with HCC patients' survival and relapse. The switch of AFMID isoforms is an early event in HCC development and is associated with driver mutations in TP53 and ARID1A. The switch of AFMID isoforms is human-specific and not detectable in other species, including primates. Finally, we show that overexpression of the full-length AFMID isoform leads to a higher NAD+ level, lower DNA-damage response, and slower cell growth in HepG2 cells. The integrative analysis uncovered a mechanistic link between splicing switches, de novo NAD+ biosynthesis, driver mutations, and HCC recurrence.

原文英語
頁(從 - 到)275-284
頁數10
期刊Genome Research
28
發行號3
DOIs
出版狀態已發佈 - 三月 1 2018

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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